Table 1 Differential expressed proteins:... | American Society of Hematology

Table 1

Differential expressed proteins: activated CD4⁺ T cells versus CD25⁺ Treg cells

No.	Protein	Gene	Accession no.	Gene locus	Expression ratio (n = 3) CD25⁺: CD4⁺
8	Galectin-10	CLC	17942629	19q13.1	42
12	Tropomyosin 3	TPM3	24119203	1q21.2	4
69	Actin, beta, cytoplasmic isoform A	ACTB	14250401	7p15-p12	3.3
14	Actin, beta cytoplasmic isoform B	ACTB	14250401	7p15-p12	3.2
15	Actin, beta cytoplasmic isoform C	ACTB	14250401	7p15-p12	2.4
42	EH domain-containing 1	EHD1	9956076	11q13	1.7
36	Galectin 1	LGALS1	4504981	22q13.1	1.6
89	Actin gamma	ACTG1	178045	17q25.3	1.4
129	Heterogeneous nuclear ribonucleoprotein A2/B1 isoform 2	HNRPA2B	4504447	7p15.2	1.2
112	Tumor protein, translationally controlled 1	TPT1	4507669	13q12-q14	0.8
135	Homeobox prox 1	PROX1	7706322	14q22.1	0.8
103	FK506-binding protein 4	FKBP4	4503729	12p13.33	0.8
26	Stathmin 1	STMN1	5031851	1p36.1-p35	0.6
2460	MO25 protein	MO25	7706481	2q37.1	0.6
22	Proteasome subunit beta type 7	PSMB7	21465650	9q34.11-q34.12	0.6
115b	Nucleoside diphosphate kinase A isoform A	NME1	4557797	17q21.3	0.6
115a	Nucleoside diphosphate kinase A isoform B	NME1	4557797	17q21.3	0.6
39	Ribonucleoside-diphosphate reductase isoform A	RRM1	4506749	11p15.5	0.5
38	Ribonucleoside-diphosphate reductase isoform B	RRM1	4506749	11p15.5	0.5
48	Flap structure-specific endonuclease 1	FEN1	4758356	11q12	0.5
3	dUTP pyrophosphatase–isoform A	DUT	4503423	15q15-q21.1	0.4
30	Eukaryotic translation initiation factor 5A	EIF5A	4503545	17p13-p12	0.3
25	dUTP pyrophosphatase–isoform B	DUT	4503423	15q15-q21.1	0.3

No.	Protein	Gene	Accession no.	Gene locus	Expression ratio (n = 3) CD25⁺: CD4⁺
8	Galectin-10	CLC	17942629	19q13.1	42
12	Tropomyosin 3	TPM3	24119203	1q21.2	4
69	Actin, beta, cytoplasmic isoform A	ACTB	14250401	7p15-p12	3.3
14	Actin, beta cytoplasmic isoform B	ACTB	14250401	7p15-p12	3.2
15	Actin, beta cytoplasmic isoform C	ACTB	14250401	7p15-p12	2.4
42	EH domain-containing 1	EHD1	9956076	11q13	1.7
36	Galectin 1	LGALS1	4504981	22q13.1	1.6
89	Actin gamma	ACTG1	178045	17q25.3	1.4
129	Heterogeneous nuclear ribonucleoprotein A2/B1 isoform 2	HNRPA2B	4504447	7p15.2	1.2
112	Tumor protein, translationally controlled 1	TPT1	4507669	13q12-q14	0.8
135	Homeobox prox 1	PROX1	7706322	14q22.1	0.8
103	FK506-binding protein 4	FKBP4	4503729	12p13.33	0.8
26	Stathmin 1	STMN1	5031851	1p36.1-p35	0.6
2460	MO25 protein	MO25	7706481	2q37.1	0.6
22	Proteasome subunit beta type 7	PSMB7	21465650	9q34.11-q34.12	0.6
115b	Nucleoside diphosphate kinase A isoform A	NME1	4557797	17q21.3	0.6
115a	Nucleoside diphosphate kinase A isoform B	NME1	4557797	17q21.3	0.6
39	Ribonucleoside-diphosphate reductase isoform A	RRM1	4506749	11p15.5	0.5
38	Ribonucleoside-diphosphate reductase isoform B	RRM1	4506749	11p15.5	0.5
48	Flap structure-specific endonuclease 1	FEN1	4758356	11q12	0.5
3	dUTP pyrophosphatase–isoform A	DUT	4503423	15q15-q21.1	0.4
30	Eukaryotic translation initiation factor 5A	EIF5A	4503545	17p13-p12	0.3
25	dUTP pyrophosphatase–isoform B	DUT	4503423	15q15-q21.1	0.3

The ratios of protein spot intensities comparing activated CD4⁺ T cells with activated CD25⁺ Treg cells are shown. Proteins from whole-cell lysates were separated by 2D gel electrophoresis. Corresponding protein spots were matched using the ProteomWeaver image analysis software, and intensities were acquired. Intensity ratios were calculated on the basis of 2D PAGE from sets of T cells derived from 3 individual human volunteers performed as triplicates.

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