Table 2

Primers for RT-PCR amplification

GeneForwardReverse
VH4 IgM 5′-ATGAAACACCTGTGGTTCTT-3′ 5′-CACGTTCTTTTCTTTGTTGC-3′ 
VH4 IgG 5′-ATGAAACACCTGTGGTTCTT-3′ 5′-AGTAGTCCTTGACCAGGCAGCCCAG-3′ 
β-actin 5′-ATCCACACGGAGTACTTGCGCTCA-3′ 5′-GTACCACTGGCATCGTGATGGACT-3′ 
CD45* 5¢-GGCAAAGCCCAACACCTT-3′ 5′-TGTGGTTGAAATGACAGCG-3′ 
AID† 5′-ACTTGCAGGGAGGCAAGAAGACACTCT-3′ 5′-CAAAAGGATGCGCCGAAGCTGTCTGGAG-3′ 
GeneForwardReverse
VH4 IgM 5′-ATGAAACACCTGTGGTTCTT-3′ 5′-CACGTTCTTTTCTTTGTTGC-3′ 
VH4 IgG 5′-ATGAAACACCTGTGGTTCTT-3′ 5′-AGTAGTCCTTGACCAGGCAGCCCAG-3′ 
β-actin 5′-ATCCACACGGAGTACTTGCGCTCA-3′ 5′-GTACCACTGGCATCGTGATGGACT-3′ 
CD45* 5¢-GGCAAAGCCCAACACCTT-3′ 5′-TGTGGTTGAAATGACAGCG-3′ 
AID† 5′-ACTTGCAGGGAGGCAAGAAGACACTCT-3′ 5′-CAAAAGGATGCGCCGAAGCTGTCTGGAG-3′ 
*

Ratech et al.43 

†

Zhou et al.32 

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