Fine epitope mapping with minigenes
| Minigene sequence . | Length, bp . | CTL response . | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M | E | I | F | I | E | V | F | S | H | F | L | L | Q | L | T | 16 | + |
| M | E | I | F | I | E | V | F | S | H | F | L | L | Q | L | 15 | + | |
| M | E | I | F | I | E | V | F | S | H | F | L | L | Q | 14 | + | ||
| M | E | I | F | I | E | V | F | S | H | F | L | L | 13 | + | |||
| M | E | I | F | I | E | V | F | S | H | F | L | 12 | + | ||||
| M | E | I | F | I | E | V | F | S | H | F | 11 | + | |||||
| M | E | I | F | I | E | V | F | S | H | 10 | − | ||||||
| M | E | I | F | I | E | V | F | S | 9 | − | |||||||
| Minigene sequence . | Length, bp . | CTL response . | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M | E | I | F | I | E | V | F | S | H | F | L | L | Q | L | T | 16 | + |
| M | E | I | F | I | E | V | F | S | H | F | L | L | Q | L | 15 | + | |
| M | E | I | F | I | E | V | F | S | H | F | L | L | Q | 14 | + | ||
| M | E | I | F | I | E | V | F | S | H | F | L | L | 13 | + | |||
| M | E | I | F | I | E | V | F | S | H | F | L | 12 | + | ||||
| M | E | I | F | I | E | V | F | S | H | F | 11 | + | |||||
| M | E | I | F | I | E | V | F | S | H | 10 | − | ||||||
| M | E | I | F | I | E | V | F | S | 9 | − | |||||||
To determine the mHA epitope, a minigene encoding 16 amino acids, which stimulated CTL-2A12, was serially deleted from its C terminus and then tested by ELISA. An undecameric but not decameric peptide was sufficient to induce IFN-γ production from the CTL-2A12.