Number of genes altered by Smad1 and/or Smad5 knockdown
| Genes . | No. (%) . |
|---|---|
| Overexpressed | |
| Smad1 knockdown | |
| Total | 702 (100) |
| Unique | 542 (77.2) |
| Overlap with Smad5 knockdown | 160 (22.8) |
| Smad5 knockdown | |
| Total | 557 (100) |
| Unique | 397 (71.3) |
| Overlap with Smad1 knockdown | 160 (28.7) |
| Smad1 + 5 knockdown | |
| Total | 742 (100) |
| Unique | 360 (48.5) |
| Underexpressed | |
| Smad1 knockdown | |
| Total | 718 (100) |
| Unique | 564 (78.6) |
| Overlap with Smad5 knockdown | 154 (21.4) |
| Smad5 knockdown | |
| Total | 461 (100) |
| Unique | 313 (67.9) |
| Overlap with Smad1 knockdown | 154 (33.4) |
| Smad 1 + 5 knockdown | |
| Total | 1006 (100) |
| Unique | 579 (57.6) |
| Genes . | No. (%) . |
|---|---|
| Overexpressed | |
| Smad1 knockdown | |
| Total | 702 (100) |
| Unique | 542 (77.2) |
| Overlap with Smad5 knockdown | 160 (22.8) |
| Smad5 knockdown | |
| Total | 557 (100) |
| Unique | 397 (71.3) |
| Overlap with Smad1 knockdown | 160 (28.7) |
| Smad1 + 5 knockdown | |
| Total | 742 (100) |
| Unique | 360 (48.5) |
| Underexpressed | |
| Smad1 knockdown | |
| Total | 718 (100) |
| Unique | 564 (78.6) |
| Overlap with Smad5 knockdown | 154 (21.4) |
| Smad5 knockdown | |
| Total | 461 (100) |
| Unique | 313 (67.9) |
| Overlap with Smad1 knockdown | 154 (33.4) |
| Smad 1 + 5 knockdown | |
| Total | 1006 (100) |
| Unique | 579 (57.6) |
For each morphant class, the total number of genes is shown that are altered by a 2-fold or higher change in transcript level, as determined by microarray. The number of genes that are unique to each set (ie, those that are not expressed in the other single knockdown), and the number that are present in both sets (overlap) are also shown. For each of the single Smad knockdowns, approximately 75% of the genes altered by MO injection at the 1-somite stage are unique to that subset. For the double-knockdown experiment, the unique genes are those that are not expressed in either of the single knockdowns, and represent genes that are regulated redundantly by Smad1 and Smad5. In this case, around 50% of the genes are unique.