Primers used for recombinant FIX virus construction
| Name . | HCMV nt . | Sequence . |
|---|---|---|
| 15kbsub1 fwd | 33979 | atg ctc aca ctc tat ctc ttc aca gcg aca tgt tgc ttc gtc tat gcg ctc agc gag tca gtg agc gag g |
| 15kbsub1 rev | 39422 | tac aac gga cgc gct cgc tgt ttc ggt ctc agg tca tct gca ttg act cgc agg cgg ccg cac tag tga t |
| 15kbsub2 fwd | 39395 | acc gaa aca gcg agc gcg tcc gtt gta cag ttg tat agc agc aca cgc ctc agc gag tca gtg agc gag g |
| 15kbsub2 rev | 45002 | ttt taa cgg cga cag tac agc tcc cgt taa aca tta gat taa tag acg ctc agg cgg ccg cac tag tga t |
| 15kbsub3 fwd | 44846 | aac aca tat act ccc cac gat cct cga ac acct tac agc ata tga gca aac agc gag tca gtg agc gag g |
| 15kbsub3 rev | 48104 | cga acg acg tgt gac gag gac gtg gtt tcc gca agc ctc tac cga cgc cgc agg cgg ccg cac tag tga t |
| 15kbsub4 fwd | 33979 | 15kbsub1 fwd primer used |
| 15kbsub4 rev | 49104 | 15kbsub4 rev primer used |
| UL136fwd | 39491 | tca agg gcg tgg aga tgc cag aaa tga cgt ggg act tgg acg ttg gaa ata cca cgt cgt gga atg cc |
| UL136rev | 40064 | tta ccc cgc cag cag cac ctc cgc cgg caa ccg cgt cgt cgt tgc tat cgt ccc atg tgc agg tgc tg |
| UL137fwd | 40210 | ggt tga ggg ggc cgt tcc cgc gcg agt gct gta caa aag aga gag act gga cca cgt cgt gga atg cc |
| UL137rev | 40285 | acg gtc ctc tgt ccg gat cta cgt ccc agt ctc tct ctt ttg tac agc act ccc atg tgc agg tgc tg |
| UL138fwd | 40360 | ctg cta tct agc tta cca ttg gca cga cac ctt caa act ggt gcg cat gta cca cgt cgt gga atg cc |
| UL138rev | 40798 | tca cgt gta ttc ttg atg ata atg tac cat ggc tac ggt ggt gaa ctg cgt ccc atg tgc agg tgc tg |
| UL139fwd | 41361 | atg ctg tgg ata tta att tta ttt gca ctc gcc gca tcg gcg agt gaa aca cca cgt cgt gga atg cc |
| UL139rev | 41768 | tca ccg agg cgg agg tgg aaa tga gcc gtc ctg tgg ggg agt gta cga cct ccc atg tgc agg tgc tg |
| UL140fwd | 41950 | atg acc cca gct cag act aac gcc act acc acc gtg cac ccg cac gac aca cca cgt cgt gga atg cc |
| UL140rev | 42525 | tca cag ggt ctg atg aag ctg cca aga gtc gtg gct gtg gcg cag cgc gtt ccc atg tgc agg tgc tg |
| UL141fwd | 42564 | atg aga cag gtc gcg tac cgc cgg cga cgc gag agt tcc tgc gcg gtg cta cca cgt cgt gga atg cc |
| UL141rev | 43840 | tca cct ctt cat ctt ttt aac acc ggg gta act atc gta agt cgg tag gct ccc atg tgc agg tgc tg |
| UL142fwd | 43913 | atg cgg att gaa tgg gcg tgt tgg tta ttc gga tac ttt gtg tca tcc gta cca cgt cgt gga atg cc |
| UL142rev | 44830 | tta ctg acc gcg cca tac ttc gta tac gaa cct aac cgg cgt aaa gtg ttt ccc atg tgc agg tgc tg |
| Name . | HCMV nt . | Sequence . |
|---|---|---|
| 15kbsub1 fwd | 33979 | atg ctc aca ctc tat ctc ttc aca gcg aca tgt tgc ttc gtc tat gcg ctc agc gag tca gtg agc gag g |
| 15kbsub1 rev | 39422 | tac aac gga cgc gct cgc tgt ttc ggt ctc agg tca tct gca ttg act cgc agg cgg ccg cac tag tga t |
| 15kbsub2 fwd | 39395 | acc gaa aca gcg agc gcg tcc gtt gta cag ttg tat agc agc aca cgc ctc agc gag tca gtg agc gag g |
| 15kbsub2 rev | 45002 | ttt taa cgg cga cag tac agc tcc cgt taa aca tta gat taa tag acg ctc agg cgg ccg cac tag tga t |
| 15kbsub3 fwd | 44846 | aac aca tat act ccc cac gat cct cga ac acct tac agc ata tga gca aac agc gag tca gtg agc gag g |
| 15kbsub3 rev | 48104 | cga acg acg tgt gac gag gac gtg gtt tcc gca agc ctc tac cga cgc cgc agg cgg ccg cac tag tga t |
| 15kbsub4 fwd | 33979 | 15kbsub1 fwd primer used |
| 15kbsub4 rev | 49104 | 15kbsub4 rev primer used |
| UL136fwd | 39491 | tca agg gcg tgg aga tgc cag aaa tga cgt ggg act tgg acg ttg gaa ata cca cgt cgt gga atg cc |
| UL136rev | 40064 | tta ccc cgc cag cag cac ctc cgc cgg caa ccg cgt cgt cgt tgc tat cgt ccc atg tgc agg tgc tg |
| UL137fwd | 40210 | ggt tga ggg ggc cgt tcc cgc gcg agt gct gta caa aag aga gag act gga cca cgt cgt gga atg cc |
| UL137rev | 40285 | acg gtc ctc tgt ccg gat cta cgt ccc agt ctc tct ctt ttg tac agc act ccc atg tgc agg tgc tg |
| UL138fwd | 40360 | ctg cta tct agc tta cca ttg gca cga cac ctt caa act ggt gcg cat gta cca cgt cgt gga atg cc |
| UL138rev | 40798 | tca cgt gta ttc ttg atg ata atg tac cat ggc tac ggt ggt gaa ctg cgt ccc atg tgc agg tgc tg |
| UL139fwd | 41361 | atg ctg tgg ata tta att tta ttt gca ctc gcc gca tcg gcg agt gaa aca cca cgt cgt gga atg cc |
| UL139rev | 41768 | tca ccg agg cgg agg tgg aaa tga gcc gtc ctg tgg ggg agt gta cga cct ccc atg tgc agg tgc tg |
| UL140fwd | 41950 | atg acc cca gct cag act aac gcc act acc acc gtg cac ccg cac gac aca cca cgt cgt gga atg cc |
| UL140rev | 42525 | tca cag ggt ctg atg aag ctg cca aga gtc gtg gct gtg gcg cag cgc gtt ccc atg tgc agg tgc tg |
| UL141fwd | 42564 | atg aga cag gtc gcg tac cgc cgg cga cgc gag agt tcc tgc gcg gtg cta cca cgt cgt gga atg cc |
| UL141rev | 43840 | tca cct ctt cat ctt ttt aac acc ggg gta act atc gta agt cgg tag gct ccc atg tgc agg tgc tg |
| UL142fwd | 43913 | atg cgg att gaa tgg gcg tgt tgg tta ttc gga tac ttt gtg tca tcc gta cca cgt cgt gga atg cc |
| UL142rev | 44830 | tta ctg acc gcg cca tac ttc gta tac gaa cct aac cgg cgt aaa gtg ttt ccc atg tgc agg tgc tg |
Bolded sequences represent the portion of the primer specific to the pKan/LacZ or pKanfrtFLAG plasmid. Other sequences represent the CMV gene-specific portion of the primer.