Table 1

Primers used for recombinant FIX virus construction

NameHCMV ntSequence
15kbsub1 fwd 33979 atg ctc aca ctc tat ctc ttc aca gcg aca tgt tgc ttc gtc tat gcg ctc agc gag tca gtg agc gag g 
15kbsub1 rev 39422 tac aac gga cgc gct cgc tgt ttc ggt ctc agg tca tct gca ttg act cgc agg cgg ccg cac tag tga t 
15kbsub2 fwd 39395 acc gaa aca gcg agc gcg tcc gtt gta cag ttg tat agc agc aca cgc ctc agc gag tca gtg agc gag g 
15kbsub2 rev 45002 ttt taa cgg cga cag tac agc tcc cgt taa aca tta gat taa tag acg ctc agg cgg ccg cac tag tga t 
15kbsub3 fwd 44846 aac aca tat act ccc cac gat cct cga ac acct tac agc ata tga gca aac agc gag tca gtg agc gag g 
15kbsub3 rev 48104 cga acg acg tgt gac gag gac gtg gtt tcc gca agc ctc tac cga cgc cgc agg cgg ccg cac tag tga t 
15kbsub4 fwd 33979 15kbsub1 fwd primer used 
15kbsub4 rev 49104 15kbsub4 rev primer used 
UL136fwd 39491 tca agg gcg tgg aga tgc cag aaa tga cgt ggg act tgg acg ttg gaa ata cca cgt cgt gga atg cc 
UL136rev 40064 tta ccc cgc cag cag cac ctc cgc cgg caa ccg cgt cgt cgt tgc tat cgt ccc atg tgc agg tgc tg 
UL137fwd 40210 ggt tga ggg ggc cgt tcc cgc gcg agt gct gta caa aag aga gag act gga cca cgt cgt gga atg cc 
UL137rev 40285 acg gtc ctc tgt ccg gat cta cgt ccc agt ctc tct ctt ttg tac agc act ccc atg tgc agg tgc tg 
UL138fwd 40360 ctg cta tct agc tta cca ttg gca cga cac ctt caa act ggt gcg cat gta cca cgt cgt gga atg cc 
UL138rev 40798 tca cgt gta ttc ttg atg ata atg tac cat ggc tac ggt ggt gaa ctg cgt ccc atg tgc agg tgc tg 
UL139fwd 41361 atg ctg tgg ata tta att tta ttt gca ctc gcc gca tcg gcg agt gaa aca cca cgt cgt gga atg cc 
UL139rev 41768 tca ccg agg cgg agg tgg aaa tga gcc gtc ctg tgg ggg agt gta cga cct ccc atg tgc agg tgc tg 
UL140fwd 41950 atg acc cca gct cag act aac gcc act acc acc gtg cac ccg cac gac aca cca cgt cgt gga atg cc 
UL140rev 42525 tca cag ggt ctg atg aag ctg cca aga gtc gtg gct gtg gcg cag cgc gtt ccc atg tgc agg tgc tg 
UL141fwd 42564 atg aga cag gtc gcg tac cgc cgg cga cgc gag agt tcc tgc gcg gtg cta cca cgt cgt gga atg cc 
UL141rev 43840 tca cct ctt cat ctt ttt aac acc ggg gta act atc gta agt cgg tag gct ccc atg tgc agg tgc tg 
UL142fwd 43913 atg cgg att gaa tgg gcg tgt tgg tta ttc gga tac ttt gtg tca tcc gta cca cgt cgt gga atg cc 
UL142rev 44830 tta ctg acc gcg cca tac ttc gta tac gaa cct aac cgg cgt aaa gtg ttt ccc atg tgc agg tgc tg 
NameHCMV ntSequence
15kbsub1 fwd 33979 atg ctc aca ctc tat ctc ttc aca gcg aca tgt tgc ttc gtc tat gcg ctc agc gag tca gtg agc gag g 
15kbsub1 rev 39422 tac aac gga cgc gct cgc tgt ttc ggt ctc agg tca tct gca ttg act cgc agg cgg ccg cac tag tga t 
15kbsub2 fwd 39395 acc gaa aca gcg agc gcg tcc gtt gta cag ttg tat agc agc aca cgc ctc agc gag tca gtg agc gag g 
15kbsub2 rev 45002 ttt taa cgg cga cag tac agc tcc cgt taa aca tta gat taa tag acg ctc agg cgg ccg cac tag tga t 
15kbsub3 fwd 44846 aac aca tat act ccc cac gat cct cga ac acct tac agc ata tga gca aac agc gag tca gtg agc gag g 
15kbsub3 rev 48104 cga acg acg tgt gac gag gac gtg gtt tcc gca agc ctc tac cga cgc cgc agg cgg ccg cac tag tga t 
15kbsub4 fwd 33979 15kbsub1 fwd primer used 
15kbsub4 rev 49104 15kbsub4 rev primer used 
UL136fwd 39491 tca agg gcg tgg aga tgc cag aaa tga cgt ggg act tgg acg ttg gaa ata cca cgt cgt gga atg cc 
UL136rev 40064 tta ccc cgc cag cag cac ctc cgc cgg caa ccg cgt cgt cgt tgc tat cgt ccc atg tgc agg tgc tg 
UL137fwd 40210 ggt tga ggg ggc cgt tcc cgc gcg agt gct gta caa aag aga gag act gga cca cgt cgt gga atg cc 
UL137rev 40285 acg gtc ctc tgt ccg gat cta cgt ccc agt ctc tct ctt ttg tac agc act ccc atg tgc agg tgc tg 
UL138fwd 40360 ctg cta tct agc tta cca ttg gca cga cac ctt caa act ggt gcg cat gta cca cgt cgt gga atg cc 
UL138rev 40798 tca cgt gta ttc ttg atg ata atg tac cat ggc tac ggt ggt gaa ctg cgt ccc atg tgc agg tgc tg 
UL139fwd 41361 atg ctg tgg ata tta att tta ttt gca ctc gcc gca tcg gcg agt gaa aca cca cgt cgt gga atg cc 
UL139rev 41768 tca ccg agg cgg agg tgg aaa tga gcc gtc ctg tgg ggg agt gta cga cct ccc atg tgc agg tgc tg 
UL140fwd 41950 atg acc cca gct cag act aac gcc act acc acc gtg cac ccg cac gac aca cca cgt cgt gga atg cc 
UL140rev 42525 tca cag ggt ctg atg aag ctg cca aga gtc gtg gct gtg gcg cag cgc gtt ccc atg tgc agg tgc tg 
UL141fwd 42564 atg aga cag gtc gcg tac cgc cgg cga cgc gag agt tcc tgc gcg gtg cta cca cgt cgt gga atg cc 
UL141rev 43840 tca cct ctt cat ctt ttt aac acc ggg gta act atc gta agt cgg tag gct ccc atg tgc agg tgc tg 
UL142fwd 43913 atg cgg att gaa tgg gcg tgt tgg tta ttc gga tac ttt gtg tca tcc gta cca cgt cgt gga atg cc 
UL142rev 44830 tta ctg acc gcg cca tac ttc gta tac gaa cct aac cgg cgt aaa gtg ttt ccc atg tgc agg tgc tg 

Bolded sequences represent the portion of the primer specific to the pKan/LacZ or pKanfrtFLAG plasmid. Other sequences represent the CMV gene-specific portion of the primer.

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