RT-PCR primers and probes
| Primer sequences . | Tm; MgCl2; product size, bp . |
|---|---|
| UL138 | 55°C; 1.5 mM; 333 |
| 5′-TGC GCA TGT TTC TGA GCT AC-3′ | |
| 5′-ACG GGT TTC AAC AGA TCG AC-3′ | |
| UL138 DNA probe | 55°C; 1.5 mM; 150 |
| 5′-GAG CTG TAC GGG GAG TAC GA-3′ | |
| 5′-AGC TGC ACT GGG AAG ACA CT-3′ | |
| UL122/123 | 55°C; 2.5 mM; 194 |
| 5′-ATT CTT CGG CCA ACT CTG GA-3′ | |
| 5′-ACA CGA TGG AGT CCT CTG CC-3′ | |
| UL122/123 DNA probe | 55°C; 2 mM; 201 |
| 5′-CCC TGA TAA TCC TGA CGA GG-3′ | |
| 5′-CAT AGT CTG CAG GAA CGT CGT-3′ | |
| GAPDH | 60°C; 3 mM; 238 |
| 5′-GAG TCA ACG GAT TTG GTC GT-3′ | |
| 5′-TTG ATT TTG GAG GGA TCT CG-3′ |
| Primer sequences . | Tm; MgCl2; product size, bp . |
|---|---|
| UL138 | 55°C; 1.5 mM; 333 |
| 5′-TGC GCA TGT TTC TGA GCT AC-3′ | |
| 5′-ACG GGT TTC AAC AGA TCG AC-3′ | |
| UL138 DNA probe | 55°C; 1.5 mM; 150 |
| 5′-GAG CTG TAC GGG GAG TAC GA-3′ | |
| 5′-AGC TGC ACT GGG AAG ACA CT-3′ | |
| UL122/123 | 55°C; 2.5 mM; 194 |
| 5′-ATT CTT CGG CCA ACT CTG GA-3′ | |
| 5′-ACA CGA TGG AGT CCT CTG CC-3′ | |
| UL122/123 DNA probe | 55°C; 2 mM; 201 |
| 5′-CCC TGA TAA TCC TGA CGA GG-3′ | |
| 5′-CAT AGT CTG CAG GAA CGT CGT-3′ | |
| GAPDH | 60°C; 3 mM; 238 |
| 5′-GAG TCA ACG GAT TTG GTC GT-3′ | |
| 5′-TTG ATT TTG GAG GGA TCT CG-3′ |
Primers are paired, with one forward and one reverse for each.
Tm indicates temperature.