Correlation of IL2RG lentiviral vector copy number with restoration of pSTAT-5 in IL2RG-defficient human ED-7R cells
| MOI . | A2UCOE-IL2RG . | SFFV-IL2RG . | ||
|---|---|---|---|---|
| Copy number/cell . | % STAT-5 . | Copy number/cell . | % STAT-5 . | |
| 0.1 | 0.11 | 28 | 0.16 | 9 |
| 0.5 | 0.28 | 54 | 0.16 | 23 |
| 1 | 0.44 | 68 | 0.25 | 40 |
| 5 | 1.2 | 77 | 0.44 | 65 |
| MOI . | A2UCOE-IL2RG . | SFFV-IL2RG . | ||
|---|---|---|---|---|
| Copy number/cell . | % STAT-5 . | Copy number/cell . | % STAT-5 . | |
| 0.1 | 0.11 | 28 | 0.16 | 9 |
| 0.5 | 0.28 | 54 | 0.16 | 23 |
| 1 | 0.44 | 68 | 0.25 | 40 |
| 5 | 1.2 | 77 | 0.44 | 65 |
Percentage of pSTAT-5–positive cells after transduction with either the A2UCOE-IL2RG or SFFV-IL2RG lentiviral vectors and stimulation with IL-2 (Figure 6A) correlated with vector copy number determined by QPCR at different MOIs. Note that at low (0.1, 0.5) MOI A2UCOE-IL2RG gives a higher proportion of pSTAT-5–positive cells than the SFFV-IL2RG vector.