Reconstitution in spleens of mice following ex vivo bone marrow HSC gene transfer with the A2UCOE-IL2RG and SFFV-IL2RG lentiviral vector
| . | CD3, % . | CD4, % . | CD8, % . | B220/IgM, % . | IgM, % . |
|---|---|---|---|---|---|
| Mock | 0.46 | 0.74 | 1.4 | 0.55 | 0.64 |
| A2UCOE-γ c 1 | 18.1 | 22.4 | 6 | 38.5 | 12.5 |
| A2UCOE-γ c 2 | 30.4 | 25.7 | 7.4 | 24.3 | 22.5 |
| A2UCOE-γ c 3 | 34 | 19.2 | 7.4 | 9.5 | 24 |
| A2UCOE-γ c 4 | 37 | 29 | 9.3 | 18.1 | 22 |
| A2UCOE-γ c 5 | 23 | 17.4 | 7.7 | 5.1 | 9.4 |
| SFFV-γ c 1 | 14.7 | 10.9 | 3.4 | 4.5 | 0 |
| SFFV-γ c 2 | 9.3 | 8.5 | 1.5 | 2.6 | 1.5 |
| Control | 15.9 | 10.9 | 6.2 | 68.6 | 6.5 |
| . | CD3, % . | CD4, % . | CD8, % . | B220/IgM, % . | IgM, % . |
|---|---|---|---|---|---|
| Mock | 0.46 | 0.74 | 1.4 | 0.55 | 0.64 |
| A2UCOE-γ c 1 | 18.1 | 22.4 | 6 | 38.5 | 12.5 |
| A2UCOE-γ c 2 | 30.4 | 25.7 | 7.4 | 24.3 | 22.5 |
| A2UCOE-γ c 3 | 34 | 19.2 | 7.4 | 9.5 | 24 |
| A2UCOE-γ c 4 | 37 | 29 | 9.3 | 18.1 | 22 |
| A2UCOE-γ c 5 | 23 | 17.4 | 7.7 | 5.1 | 9.4 |
| SFFV-γ c 1 | 14.7 | 10.9 | 3.4 | 4.5 | 0 |
| SFFV-γ c 2 | 9.3 | 8.5 | 1.5 | 2.6 | 1.5 |
| Control | 15.9 | 10.9 | 6.2 | 68.6 | 6.5 |
SCID-X1 mice were subjected to an ex vivo procedure with HSCs transduced with the A2UCOE-IL2RG lentiviral vector (Figure 1B). At 3 months following engraftment, spleens were excised and analyzed for reconstitution of T-, B-, and NK cell lineages by FACS. Cells were stained with anti-CD8, -CD4, -NK1.1, -IgM, and -B220 antibodies. Efficient reconstitution of all cell lineages is observed in A2UCOE-IL2RG vector and SFFV-IL2RG–positive control transduced mice.