Amino acid sequences of synthetic peptides
| Peptide . | Sequence . | Tm (°C) . |
|---|---|---|
| CRP | GCO(GPO)10GCOG-NH2 | 82.3 |
| GPP | GPC(GPP)10GPC-NH2 | 4111 |
| GFOGER | GPC(GPP)5GFOGER(GPP)5GPC-NH2 | 43.927 |
| III-01 | GPC(GPP)5GLAGYOGPAGPOGPOGPOGTSGHOGSO(GPP)5GPC-NH2 | 41.918 |
| III-01a | GPC(GPP)5GLAGYOGPAGPOGPOGPOGTSGPOGPO(GPP)5GPC-NH2 | 59.0 |
| III-01b | GPC(GPP)5GLAGYOGPAGPOGPPGPPGTSGPOGPO(GPP)5GPC-NH2 | 55.0 |
| III-01c | GPC(GPP)5GPOGYOGPAGPPGPOGPOGTSGHOGSO(GPP)5GPC-NH2 | 55.1 |
| III-30 | GPC(GPP)5GAOGLRGGAGPOGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 42.918 |
| III-30a | GPC(GPP)5GAOGLRGGAGPOGPEGGKGAAGPOGPP(GPP)5GPC-NH2 | 44.7 |
| III-30b | GPC(GPP)5GAOGLRGGAGPOGPEGGKGAAGPPGPO(GPP)5GPC-NH2 | 45.1 |
| III-30c | GPC(GPP)5GAOGLRGGAGPPGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 43.9 |
| p1 | GPC(GPP)5GPQGLQGLOGTSGPOGEN(GPP)5GPC-NH2 | 43.5 |
| p2 | GPC(GPP)5GTSGPOGENGKOGEOGPK(GPP)5GPC-NH2 | 43.6 |
| p3 | GPC(GPP)5GKOGEOGPKGEAGAOGIO(GPP)5GPC-NH2 | 51.5 |
| p4 | GPC(GPP)5GEAGAOGIOGGKGDSGAO(GPP)5GPC-NH2 | 38.2 |
| p5 | GPC(GPP)5GGKGDSGAOGERGPOGAG(GPP)5GPC-NH2 | 34.6 |
| p6 | GPC(GPP)5GERGPOGAGGPOGPRGGA(GPP)5GPC-NH2 | 18.1 |
| p7 | GPC(GPP)5GPOGPRGGAGPOGPEGGK(GPP)5GPC-NH2 | 49.9 |
| p8 | GPC(GPP)5GPOGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 54.2 |
| p9 | GPC(GPP)5GAAGPOGPOGSAGTOGLQ(GPP)5GPC-NH2 | 50.8 |
| p8-A534 | GPC(GPP)5GPAGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 49.8 |
| p8-A546 | GPC(GPP)5GPOGPEGGKGAAGPAGPO(GPP)5GPC-NH2 | 50.9 |
| p8-A549 | GPC(GPP)5GPOGPEGGKGAAGPOGPA(GPP)5GPC-NH2 | 51.1 |
| p8-A546/549 | GPC(GPP)5GPOGPEGGKGAAGPAGPA(GPP)5GPC-NH2 | 43.8 |
| Peptide . | Sequence . | Tm (°C) . |
|---|---|---|
| CRP | GCO(GPO)10GCOG-NH2 | 82.3 |
| GPP | GPC(GPP)10GPC-NH2 | 4111 |
| GFOGER | GPC(GPP)5GFOGER(GPP)5GPC-NH2 | 43.927 |
| III-01 | GPC(GPP)5GLAGYOGPAGPOGPOGPOGTSGHOGSO(GPP)5GPC-NH2 | 41.918 |
| III-01a | GPC(GPP)5GLAGYOGPAGPOGPOGPOGTSGPOGPO(GPP)5GPC-NH2 | 59.0 |
| III-01b | GPC(GPP)5GLAGYOGPAGPOGPPGPPGTSGPOGPO(GPP)5GPC-NH2 | 55.0 |
| III-01c | GPC(GPP)5GPOGYOGPAGPPGPOGPOGTSGHOGSO(GPP)5GPC-NH2 | 55.1 |
| III-30 | GPC(GPP)5GAOGLRGGAGPOGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 42.918 |
| III-30a | GPC(GPP)5GAOGLRGGAGPOGPEGGKGAAGPOGPP(GPP)5GPC-NH2 | 44.7 |
| III-30b | GPC(GPP)5GAOGLRGGAGPOGPEGGKGAAGPPGPO(GPP)5GPC-NH2 | 45.1 |
| III-30c | GPC(GPP)5GAOGLRGGAGPPGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 43.9 |
| p1 | GPC(GPP)5GPQGLQGLOGTSGPOGEN(GPP)5GPC-NH2 | 43.5 |
| p2 | GPC(GPP)5GTSGPOGENGKOGEOGPK(GPP)5GPC-NH2 | 43.6 |
| p3 | GPC(GPP)5GKOGEOGPKGEAGAOGIO(GPP)5GPC-NH2 | 51.5 |
| p4 | GPC(GPP)5GEAGAOGIOGGKGDSGAO(GPP)5GPC-NH2 | 38.2 |
| p5 | GPC(GPP)5GGKGDSGAOGERGPOGAG(GPP)5GPC-NH2 | 34.6 |
| p6 | GPC(GPP)5GERGPOGAGGPOGPRGGA(GPP)5GPC-NH2 | 18.1 |
| p7 | GPC(GPP)5GPOGPRGGAGPOGPEGGK(GPP)5GPC-NH2 | 49.9 |
| p8 | GPC(GPP)5GPOGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 54.2 |
| p9 | GPC(GPP)5GAAGPOGPOGSAGTOGLQ(GPP)5GPC-NH2 | 50.8 |
| p8-A534 | GPC(GPP)5GPAGPEGGKGAAGPOGPO(GPP)5GPC-NH2 | 49.8 |
| p8-A546 | GPC(GPP)5GPOGPEGGKGAAGPAGPO(GPP)5GPC-NH2 | 50.9 |
| p8-A549 | GPC(GPP)5GPOGPEGGKGAAGPOGPA(GPP)5GPC-NH2 | 51.1 |
| p8-A546/549 | GPC(GPP)5GPOGPEGGKGAAGPAGPA(GPP)5GPC-NH2 | 43.8 |
Melting temperatures (Tms) were determined as previously described.18
OGP/GPO motifs are highlighted in bold, and the overlap regions between the α1(III)CB4 peptides are underlined. The 2 “GG” motifs in peptide p6 may be responsible for its low melting temperature.