Sequences of primers employed in quantitative RT-PCR
| Gene . | Primer sequence . |
|---|---|
| Nkx2.5 | GACAAAGCCGAGACGGATGG |
| CTGTCGCTTGCACTTGTAGC | |
| Gata1 | CATTGGCCCCTTGTGAGGCCAG |
| CGCTCCAGCCAGATTCGACCC | |
| Gata2 | CTGCAACACACCACCCGATA |
| GGAGCGAGCCTTGCTTCTC | |
| Gata3 | CTGACGGAAGAGGTGGACGTACTTT |
| TAGCCCTGACGGAGTTTCCGTAGTA | |
| Pax6 | AGCCCGTCAGTGAATGGGCGGAGTTAT |
| TGGCCTCGATTACAGTAAAGAGAGAAGGAG | |
| LMO2 | GGA TCC TCG GCC ATC GAA AGG AAG AGC |
| ATC CCA TTG ATC TTG GTC CAC TC | |
| PU.1 | ATG GAA GGG TTT TCC CTC ACC GCC |
| GTC CAC GCT CTG CAG CTC TGT GAA | |
| HNF439 | ACA CGT CCC CAT CTG AAG GTG |
| CTT CCT TCT TCA TGC CAG CCC | |
| Cdx1 | GTA AGA CCC GAA CCA AGG AC |
| GGA ACC AGA TCT TTA CCT GC | |
| Cdx2 | CAG TCC CTA GGA AGC CAA GTG AAA |
| AAG TGA AAC TCC TTC TCC AGC TCC | |
| Cdx4 | GAG GAA GTC AGA GCT GGC AGT TA |
| GGC TCT GCG ATT CTG AAA CC | |
| HoxA2 | CGGCCACAAAGAATCCCTGGAAAT |
| AAAGCTGAGTGTTGGTGTACGCGGTT | |
| HoxA4 | TTATAACGGAGGCGAACCTAAGCG |
| GGT CAG GTA GCG GTT AAA GTG GAA | |
| HoxA613,40 | CCG AGC AGC AGT ACA AAC CTG |
| TGC CTT CCT CAT GGA GGG | |
| HoxA713,40 | TTT CCG CAT CTA CCC CTG G |
| TAG GTC TGG CGT CCC CG | |
| HoxA913,40 | CCG AAA ACA ATG CCG AGA AT |
| CCG GGT TAT TGG GAT CGA T | |
| HoxA1013,40 | TCG CCG GAG AAG GAC TCC |
| TTT GCT GTG AGC CAG TTG G | |
| HoxA13 | CTT CGC CGA CAA GTA CAT GGA CAC |
| CGT AGC CCT GAT GGT AGA AAG CAA | |
| HoxB1 | AAG GAA TCG CCT TGC TCG TCA GAA |
| ACC TTC GCT GTC TTA GGT GGG TTT | |
| HoxB4 | CCTGGATGCGCAAAGTTCA |
| CTTGGGCTCCCCGCC | |
| HoxB513,40 | TCC CCT GGA TGA GGA AGC TT |
| CGG GCC CTT TTT CCG T | |
| HoxB613,40 | CGTCTACCCGTGGATGCAG |
| TCGCCGACCGCTGG | |
| HoxB713,40 | TTG GCG GCC GAG AGT AAC |
| TCG GTC AGG CCC TGA GC | |
| HoxB8 | GACTCGCAAGCGGAGGATC |
| GAACCAGATTTTGACCTGTCTCTCT | |
| HoxB9 | ACC AAA TAC CAG ACG CTG GAG CTA |
| TCA GAT TGA GGA GTC TGG CCA CTT |
| Gene . | Primer sequence . |
|---|---|
| Nkx2.5 | GACAAAGCCGAGACGGATGG |
| CTGTCGCTTGCACTTGTAGC | |
| Gata1 | CATTGGCCCCTTGTGAGGCCAG |
| CGCTCCAGCCAGATTCGACCC | |
| Gata2 | CTGCAACACACCACCCGATA |
| GGAGCGAGCCTTGCTTCTC | |
| Gata3 | CTGACGGAAGAGGTGGACGTACTTT |
| TAGCCCTGACGGAGTTTCCGTAGTA | |
| Pax6 | AGCCCGTCAGTGAATGGGCGGAGTTAT |
| TGGCCTCGATTACAGTAAAGAGAGAAGGAG | |
| LMO2 | GGA TCC TCG GCC ATC GAA AGG AAG AGC |
| ATC CCA TTG ATC TTG GTC CAC TC | |
| PU.1 | ATG GAA GGG TTT TCC CTC ACC GCC |
| GTC CAC GCT CTG CAG CTC TGT GAA | |
| HNF439 | ACA CGT CCC CAT CTG AAG GTG |
| CTT CCT TCT TCA TGC CAG CCC | |
| Cdx1 | GTA AGA CCC GAA CCA AGG AC |
| GGA ACC AGA TCT TTA CCT GC | |
| Cdx2 | CAG TCC CTA GGA AGC CAA GTG AAA |
| AAG TGA AAC TCC TTC TCC AGC TCC | |
| Cdx4 | GAG GAA GTC AGA GCT GGC AGT TA |
| GGC TCT GCG ATT CTG AAA CC | |
| HoxA2 | CGGCCACAAAGAATCCCTGGAAAT |
| AAAGCTGAGTGTTGGTGTACGCGGTT | |
| HoxA4 | TTATAACGGAGGCGAACCTAAGCG |
| GGT CAG GTA GCG GTT AAA GTG GAA | |
| HoxA613,40 | CCG AGC AGC AGT ACA AAC CTG |
| TGC CTT CCT CAT GGA GGG | |
| HoxA713,40 | TTT CCG CAT CTA CCC CTG G |
| TAG GTC TGG CGT CCC CG | |
| HoxA913,40 | CCG AAA ACA ATG CCG AGA AT |
| CCG GGT TAT TGG GAT CGA T | |
| HoxA1013,40 | TCG CCG GAG AAG GAC TCC |
| TTT GCT GTG AGC CAG TTG G | |
| HoxA13 | CTT CGC CGA CAA GTA CAT GGA CAC |
| CGT AGC CCT GAT GGT AGA AAG CAA | |
| HoxB1 | AAG GAA TCG CCT TGC TCG TCA GAA |
| ACC TTC GCT GTC TTA GGT GGG TTT | |
| HoxB4 | CCTGGATGCGCAAAGTTCA |
| CTTGGGCTCCCCGCC | |
| HoxB513,40 | TCC CCT GGA TGA GGA AGC TT |
| CGG GCC CTT TTT CCG T | |
| HoxB613,40 | CGTCTACCCGTGGATGCAG |
| TCGCCGACCGCTGG | |
| HoxB713,40 | TTG GCG GCC GAG AGT AAC |
| TCG GTC AGG CCC TGA GC | |
| HoxB8 | GACTCGCAAGCGGAGGATC |
| GAACCAGATTTTGACCTGTCTCTCT | |
| HoxB9 | ACC AAA TAC CAG ACG CTG GAG CTA |
| TCA GAT TGA GGA GTC TGG CCA CTT |