Effect of recombinant cytokines and HS5 CM on MMP-9 gene expression and protein secretion in CD14+ cells
| Concentration, ng/mL . | MMP-9 gene expression . | MMP-9 protein secretion . |
|---|---|---|
| HS5 CM* | 500 | 8756 |
| MCP-1/CCL2 | ||
| 0.05 | 236 | 153 |
| 0.5 | 409 | 1089 |
| 5 | 263 | 136 |
| IL-1β | ||
| 0.005 | 211 | 95 |
| 0.05 | 312 | 155 |
| 0.5 | 356 | 576 |
| IL-6 | ||
| 0.5 | 146 | 119 |
| 5 | 244 | 124 |
| 50 | 206 | 121 |
| 500 | 161 | 205 |
| M-CSF | ||
| 0.05 | 180 | 163 |
| 0.5 | 152 | 287 |
| G-CSF, 10 | 193 | ND |
| GM-CSF, 100 | 59 | ND |
| MGSA/CXCL1, 100 | 111 | ND |
| IL-8/CXCL8, 10 | 125 | ND |
| SDF-1a/CXCL12, 100 | 125 | ND |
| Concentration, ng/mL . | MMP-9 gene expression . | MMP-9 protein secretion . |
|---|---|---|
| HS5 CM* | 500 | 8756 |
| MCP-1/CCL2 | ||
| 0.05 | 236 | 153 |
| 0.5 | 409 | 1089 |
| 5 | 263 | 136 |
| IL-1β | ||
| 0.005 | 211 | 95 |
| 0.05 | 312 | 155 |
| 0.5 | 356 | 576 |
| IL-6 | ||
| 0.5 | 146 | 119 |
| 5 | 244 | 124 |
| 50 | 206 | 121 |
| 500 | 161 | 205 |
| M-CSF | ||
| 0.05 | 180 | 163 |
| 0.5 | 152 | 287 |
| G-CSF, 10 | 193 | ND |
| GM-CSF, 100 | 59 | ND |
| MGSA/CXCL1, 100 | 111 | ND |
| IL-8/CXCL8, 10 | 125 | ND |
| SDF-1a/CXCL12, 100 | 125 | ND |
Data are represented as percent control. Control values were derived from CD14 cells cultured for 5 days in control media consisting of 10% FCS in RPMI 1640 medium.
ND indicates not determined.
HS5 CM (conditioned medium) was diluted with an equal volume of fresh medium for use in culturing CD14+ cells. Concentrations of the cytokines in HS5 CM were: IL-1β, 0.25 ng/mL; MCP-1, 0.5 ng/mL; M-CSF, 0.25 ng/mL; and IL-6, 250 ng/mL. CD14 cells were harvested for RNA and supernatants collected for protein detection after 5 days of culture.