CRLF2 expression, rearrangement, and association with genomic alterations
| Case no. . | CRLF2 Expression: U133 Plus 2.0 . | FISH/PCR detection of CRLF2 genomic rearrangements . | Deletions/mutations/expression patterns . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| CRLF2 break-apart probe . | IGH@-CRLF2 . | IGH@-CRLF2 cells, percentage . | P2RY8-CRLF2 . | CRLF2 rearrangement . | BCR-ABL1–like . | JAK mutation . | IKZF1 alteration . | ||
| 9906_258 | 7020 | Positive | Positive | 96 | Negative | IGH-CRLF2 | Yes | Negative | Yes |
| 9906_234 | 6504 | Positive | Positive | 98 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_161 | 5876 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_020 | 5841 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | Yes | JAK2 R867Q | No |
| 9906_038 | 4793 | Positive | Positive | 96 | Negative | IGH-CRLF2 | Yes | JAK2 I682F | Yes |
| 9906_047* | 4471 | Positive | Positive | 80 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_222 | 4005 | Positive: deletion | Negative | 0 | ND2 | P2RY8-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_257 | 3664 | Positive | Positive | 99 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_205 | 3445 | Positive | Positive | 87 | Negative | IGH-CRLF2 | Yes | ND | Yes |
| 9906_151* | 2406 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | Yes | JAK2 QGinsR683 | Yes |
| 9906_064 | 2379 | Positive | Positive | 96 | Negative | IGH-CRLF2 | Yes | Negative | Yes |
| 9906_113 | 2373 | Positive | Negative | 0 | Negative | Unknown partner | Yes | JAK2 R683G | Yes |
| 9906_174 | 2366 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | JAK2 P933R | Yes |
| 9906_052 | 2320 | Positive | Positive | 63 | Negative | IGH-CRLF2 | No | JAK1 V658F | No |
| 9906_215 | 2258 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | Negative | Yes |
| 9906_090 | 2177 | Positive | Positive | 97 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_196 | 2145 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | Negative | Yes |
| 9906_144 | 2127 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | Yes | JAK1 L624_R629> W | No |
| 9906_217 | 1919 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | Negative | Yes |
| 9906_168 | 1721 | Positive | Positive | 90 | Negative | IGH-CRLF2 | Yes | JAK2 R683S | Yes |
| 9906_030 | 1540 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | Yes | Negative | Yes |
| 9906_192 | 1427 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | JAK2 D873N | Yes |
| 9906_200 | 1244 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | ND | ND |
| 9906_225 | 1237 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_110 | 1235 | Positive: deletion | Negative | 0 | ND | P2RY8-CRLF2 | No | JAK2 R683G | No |
| 9906_048 | 1229 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | Negative | Yes |
| 9906_170 | 1217 | Positive: deletion | Positive | > 90 | ND | P2RY8 & IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_109 | 1035 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | ND | Yes |
| 9906_094 | 744 | Positive | Positive | 67 | Negative | IGH-CRLF2 | No | Negative | No |
| Case no. . | CRLF2 Expression: U133 Plus 2.0 . | FISH/PCR detection of CRLF2 genomic rearrangements . | Deletions/mutations/expression patterns . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| CRLF2 break-apart probe . | IGH@-CRLF2 . | IGH@-CRLF2 cells, percentage . | P2RY8-CRLF2 . | CRLF2 rearrangement . | BCR-ABL1–like . | JAK mutation . | IKZF1 alteration . | ||
| 9906_258 | 7020 | Positive | Positive | 96 | Negative | IGH-CRLF2 | Yes | Negative | Yes |
| 9906_234 | 6504 | Positive | Positive | 98 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_161 | 5876 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_020 | 5841 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | Yes | JAK2 R867Q | No |
| 9906_038 | 4793 | Positive | Positive | 96 | Negative | IGH-CRLF2 | Yes | JAK2 I682F | Yes |
| 9906_047* | 4471 | Positive | Positive | 80 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_222 | 4005 | Positive: deletion | Negative | 0 | ND2 | P2RY8-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_257 | 3664 | Positive | Positive | 99 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_205 | 3445 | Positive | Positive | 87 | Negative | IGH-CRLF2 | Yes | ND | Yes |
| 9906_151* | 2406 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | Yes | JAK2 QGinsR683 | Yes |
| 9906_064 | 2379 | Positive | Positive | 96 | Negative | IGH-CRLF2 | Yes | Negative | Yes |
| 9906_113 | 2373 | Positive | Negative | 0 | Negative | Unknown partner | Yes | JAK2 R683G | Yes |
| 9906_174 | 2366 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | JAK2 P933R | Yes |
| 9906_052 | 2320 | Positive | Positive | 63 | Negative | IGH-CRLF2 | No | JAK1 V658F | No |
| 9906_215 | 2258 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | Negative | Yes |
| 9906_090 | 2177 | Positive | Positive | 97 | Negative | IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_196 | 2145 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | Negative | Yes |
| 9906_144 | 2127 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | Yes | JAK1 L624_R629> W | No |
| 9906_217 | 1919 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | Negative | Yes |
| 9906_168 | 1721 | Positive | Positive | 90 | Negative | IGH-CRLF2 | Yes | JAK2 R683S | Yes |
| 9906_030 | 1540 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | Yes | Negative | Yes |
| 9906_192 | 1427 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | JAK2 D873N | Yes |
| 9906_200 | 1244 | Positive | Positive | > 90 | Negative | IGH-CRLF2 | No | ND | ND |
| 9906_225 | 1237 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_110 | 1235 | Positive: deletion | Negative | 0 | ND | P2RY8-CRLF2 | No | JAK2 R683G | No |
| 9906_048 | 1229 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | Negative | Yes |
| 9906_170 | 1217 | Positive: deletion | Positive | > 90 | ND | P2RY8 & IGH-CRLF2 | Yes | JAK2 R683G | Yes |
| 9906_109 | 1035 | Positive: deletion | Negative | 0 | Positive | P2RY8-CRLF2 | No | ND | Yes |
| 9906_094 | 744 | Positive | Positive | 67 | Negative | IGH-CRLF2 | No | Negative | No |
FISH/PCR indicates fluorescence in situ hybridization/polymerase chain reaction; and ND, not determined.
Down syndrome.