Sequences of primers used for quantitative RT-PCR
| Gene . | Forward primer . | Reverse primer . |
|---|---|---|
| Smad4 | GTTCAGGTAGGAGAGACGTTTAAGGT | CCTTTACATTCCAACTGCACTCCT |
| Hepc | CCTATCTCCATCAACAGATG | AACAGATACCACACTGGGAA |
| Fpn | AAGGATTGACCAGCTAACCAACA | CAGCCAATGACTGGAGAACCA |
| Dcytb | GCAGCGGGCTCGAGTTTA | TTCCAGGTCCATGGCAGTCT |
| Dmt1 | AACCAACAAGCAGGTGGTTGA | CTTTGTAGATGTCCACAGCCA |
| Tf | TTGTGCCATCCCATCACAAC | CTAGTGTCCGATGCCTTCACC |
| Heph | TTGTCTCATGAAGAACATTACAGCAC | CATATGGCAATCAAAGCAGAAGA |
| Hfe | CTGAAAGGGTGGGACTACATGTTC | GGACACCACTCCCAACTTCGT |
| Tfr1 | CAGAAAGTTCCTCAGCTCAACCA | GTTCAATTCAACGTCATGGGTAAG |
| Tfr2 | AGCTGGGACGGAGGTGACTT | TCCAGGCTCACGTACACAACA |
| Sft | CTGTGCTCATTGAAGAGGACCTT | TCTGGTTGCTTTCTCAGTCACG |
| Gene . | Forward primer . | Reverse primer . |
|---|---|---|
| Smad4 | GTTCAGGTAGGAGAGACGTTTAAGGT | CCTTTACATTCCAACTGCACTCCT |
| Hepc | CCTATCTCCATCAACAGATG | AACAGATACCACACTGGGAA |
| Fpn | AAGGATTGACCAGCTAACCAACA | CAGCCAATGACTGGAGAACCA |
| Dcytb | GCAGCGGGCTCGAGTTTA | TTCCAGGTCCATGGCAGTCT |
| Dmt1 | AACCAACAAGCAGGTGGTTGA | CTTTGTAGATGTCCACAGCCA |
| Tf | TTGTGCCATCCCATCACAAC | CTAGTGTCCGATGCCTTCACC |
| Heph | TTGTCTCATGAAGAACATTACAGCAC | CATATGGCAATCAAAGCAGAAGA |
| Hfe | CTGAAAGGGTGGGACTACATGTTC | GGACACCACTCCCAACTTCGT |
| Tfr1 | CAGAAAGTTCCTCAGCTCAACCA | GTTCAATTCAACGTCATGGGTAAG |
| Tfr2 | AGCTGGGACGGAGGTGACTT | TCCAGGCTCACGTACACAACA |
| Sft | CTGTGCTCATTGAAGAGGACCTT | TCTGGTTGCTTTCTCAGTCACG |
Smad4 indicates small mutants (C elegans) and mothers against decapentaplegic homolog 4 (Drosophila); Hepc, hepcidin; Fpn, ferroportin; Dcytb, cytochrome b reductase 1; Dmt1, divalent metal transporter 1; Tf, transferrin; Heph, hephaestin; Hfe, major histocompatibility complex class I–like protein; Tfr1, transferrin receptor 1; Tfr2, transferrin receptor 2; and Sft, stimulator of Fe transport.