Estimation of duration of cell-cycle phases in 606HS2-neo, 606HS2-EzY145F, and 606HS2-EzWT
| Duration . | 606HS2-neo . | 606HS2-EzY145F . | 606HS2-EzWT . |
|---|---|---|---|
| T S, h | 9.8 ± 1.2 | 15.1 ± 0.5 | 10.1 ± 2.0 |
| T G2/M, h | 0.9 ± 0.1 | 2.0 ± 0.2 | 1.1 ± 0.2 |
| Tpot, h | 13.3 ± 1.0 | 23.7 ± 1.6 | 13.8 ± 1.7 |
| Duration . | 606HS2-neo . | 606HS2-EzY145F . | 606HS2-EzWT . |
|---|---|---|---|
| T S, h | 9.8 ± 1.2 | 15.1 ± 0.5 | 10.1 ± 2.0 |
| T G2/M, h | 0.9 ± 0.1 | 2.0 ± 0.2 | 1.1 ± 0.2 |
| Tpot, h | 13.3 ± 1.0 | 23.7 ± 1.6 | 13.8 ± 1.7 |
Duration in hours of S (T S) and G2/M (T G2/M) phases and potential doubling time (Tpot) were calculated for each experiment after a BrdU pulse (3 nM for 15 minutes) followed by a chase of 3 hours. Values represent means plus or minus SD of 3 independent experiments.