LILRB1 ligation during cellular differentiation results in a distinct and LPS-resistant DC phenotypic profile
| Marker . | Isotype . | Isotype + LPS . | LILRB1 . | LILRB1 + LPS . | ||||
|---|---|---|---|---|---|---|---|---|
| %* . | MFI† . | %* . | MFI† . | %* . | MFI† . | %* . | MFI† . | |
| CD1a | 46.5 | 45.7 | 41.3 | 30.9 | 4.6 | 16.4 | 3.7 | 15.5 |
| CD11c | 99.8 | 124.0 | 99.5 | 148.9 | 95.2 | 94.2 | 94.7 | 96.3 |
| CD14 | 27.2 | 36.5 | 26.5 | 37.8 | 90.3 | 210.1 | 90.7 | 210.4 |
| CD38 | 25.7 | 13.0 | 86.2 | 29.1 | 81.4 | 32.4 | 82.1 | 33.3 |
| CD40 | 21.5 | 32.0 | 42.4 | 39.2 | 28.6 | 19.3 | 30.9 | 18.5 |
| CD63 | 73.4 | 20.6 | 32.7 | 15.8 | 70.9 | 17.7 | 75.4 | 17.9 |
| CD80 | 72.8 | 29.3 | 97.9 | 103.7 | 98.0 | 66.7 | 98.4 | 79.3 |
| CD83 | 10.5 | 15.6 | 78.1 | 22.3 | 0.7 | 15.2 | 1.3 | 15.0 |
| CD86 | 74.8 | 58.7 | 97.8 | 80.1 | 43.8 | 46.8 | 43.8 | 43.5 |
| CCR7 | 9.3 | 19.4 | 21.1 | 26.0 | 22.2 | 28.4 | 26.7 | 22.1 |
| HLA-DR | 99.4 | 294.7 | 99.8 | 666.2 | 99.4 | 211.2 | 99.8 | 230.3 |
| Marker . | Isotype . | Isotype + LPS . | LILRB1 . | LILRB1 + LPS . | ||||
|---|---|---|---|---|---|---|---|---|
| %* . | MFI† . | %* . | MFI† . | %* . | MFI† . | %* . | MFI† . | |
| CD1a | 46.5 | 45.7 | 41.3 | 30.9 | 4.6 | 16.4 | 3.7 | 15.5 |
| CD11c | 99.8 | 124.0 | 99.5 | 148.9 | 95.2 | 94.2 | 94.7 | 96.3 |
| CD14 | 27.2 | 36.5 | 26.5 | 37.8 | 90.3 | 210.1 | 90.7 | 210.4 |
| CD38 | 25.7 | 13.0 | 86.2 | 29.1 | 81.4 | 32.4 | 82.1 | 33.3 |
| CD40 | 21.5 | 32.0 | 42.4 | 39.2 | 28.6 | 19.3 | 30.9 | 18.5 |
| CD63 | 73.4 | 20.6 | 32.7 | 15.8 | 70.9 | 17.7 | 75.4 | 17.9 |
| CD80 | 72.8 | 29.3 | 97.9 | 103.7 | 98.0 | 66.7 | 98.4 | 79.3 |
| CD83 | 10.5 | 15.6 | 78.1 | 22.3 | 0.7 | 15.2 | 1.3 | 15.0 |
| CD86 | 74.8 | 58.7 | 97.8 | 80.1 | 43.8 | 46.8 | 43.8 | 43.5 |
| CCR7 | 9.3 | 19.4 | 21.1 | 26.0 | 22.2 | 28.4 | 26.7 | 22.1 |
| HLA-DR | 99.4 | 294.7 | 99.8 | 666.2 | 99.4 | 211.2 | 99.8 | 230.3 |
Cell populations cultured in the presence of anti-LILRB1 mAb or isotype control and subsequently exposed or not to LPS, as indicated, were analyzed by flow cytometry for expression of the indicated markers.
Percentage of positive cells.
Geometric mean fluorescence of positive cells. Results are representative of more than 12 experiments.