Inhibition of NK cell chemotaxis by anti-CXCR1 and CXCR2 Ab treatment of reovirus-CBMC supernatants
| Chemotaxis sample . | No treatment . | Anti-CXCR1 . | Isotype control (low dose) . | Anti-CXCR1 plus anti-CXCR2 . | Isotype control (high dose) . |
|---|---|---|---|---|---|
| CBMC medium | 8.5 ± 1.5 | ND | ND | 7.2 ± 1.2 | 7.7 ± 1.0 |
| CBMC reovirus | 18.5 ± 2.7* | 8.7 ± 1.1† | 16.2 ± 1.8 | 9.8 ± 1.1‡ | 16.4 ± 2.2 |
| CBMC UV-reovirus | 11.1 ± 1.0 | 6.7 ± 0.6† | 9.8 ± 0.7‡ | 6.5 ± 0.6‡ | 9.6 ± 1.0 |
| Chemotaxis sample . | No treatment . | Anti-CXCR1 . | Isotype control (low dose) . | Anti-CXCR1 plus anti-CXCR2 . | Isotype control (high dose) . |
|---|---|---|---|---|---|
| CBMC medium | 8.5 ± 1.5 | ND | ND | 7.2 ± 1.2 | 7.7 ± 1.0 |
| CBMC reovirus | 18.5 ± 2.7* | 8.7 ± 1.1† | 16.2 ± 1.8 | 9.8 ± 1.1‡ | 16.4 ± 2.2 |
| CBMC UV-reovirus | 11.1 ± 1.0 | 6.7 ± 0.6† | 9.8 ± 0.7‡ | 6.5 ± 0.6‡ | 9.6 ± 1.0 |
Data are presented as the mean plus or minus SEM of the percentage migration of the total input NK cell population of 4 separate experiments.
ND indicates not determined.
P < .05, compared with CBMC medium control.
P < .01, compared with no antibody treatment.
P < .05, compared with no antibody treatment.