Effect of anti-cytokine antibody absorptions on TTP plasma-mediated suppression of c-FLIP protein expression in dermal MVECs
| Anticytokine antibody used for plasma absorption . | c-FLIP protein, density units* . | Inhibition of TTP plasma effect on c-FLIP expression, % . | ||
|---|---|---|---|---|
| Control . | TTP plasma . | Absorbed TTP plasma . | ||
| TRAIL, experiment 1 | 8834 | 767 | 5350 | 56.8 |
| TRAIL, experiment 2 | 8834 | 2169 | 6937 | 71.5 |
| IFN-γ | 3744 | 2757 | 3492 | 74.6 |
| TNF-α | 3744 | 2757 | 2520 | 0 |
| IL-6 | 3744 | 2757 | 2946 | 19.3 |
| Anticytokine antibody used for plasma absorption . | c-FLIP protein, density units* . | Inhibition of TTP plasma effect on c-FLIP expression, % . | ||
|---|---|---|---|---|
| Control . | TTP plasma . | Absorbed TTP plasma . | ||
| TRAIL, experiment 1 | 8834 | 767 | 5350 | 56.8 |
| TRAIL, experiment 2 | 8834 | 2169 | 6937 | 71.5 |
| IFN-γ | 3744 | 2757 | 3492 | 74.6 |
| TNF-α | 3744 | 2757 | 2520 | 0 |
| IL-6 | 3744 | 2757 | 2946 | 19.3 |
Dermal MVECs were exposed to control plasma or TTP patient plasma, either untreated or preabsorbed with the indicated polyclonal anticytokine antibody. c-FLIP and control GAPDH levels were assessed by western blotting 18 hours after plasma exposure.
Level of c-FLIP in dermal MVECs exposed to various plasmas expressed as density units normalized to GAPDH.