Diagnostic criteria for primary myelofibrosis
| Leukoerythroblastic blood picture |
| Increased marrow reticulin in the absence of an infiltrative or granulomatous process |
| Splenomegaly |
| JAK2 V617F assay (peripheral blood; expression establishes the presence of an MPD but not its type; PV is always a consideration; absence does not exclude an MPD) |
| Increased circulating CD34+ cells (> 15 × 106/L) and no increase in marrow CD34+ cells by in situ immunohistochemistry |
| Characteristic cytogenetic abnormalities (peripheral blood: del(13q), 9p, del(20q), del(12p), partial trisomy1q, trisomy 8, and trisomy 9) |
| Absence of Bcr-Abl, AML, or MDS cytogenetic abnormalities by FISH (peripheral blood) |
| Leukoerythroblastic blood picture |
| Increased marrow reticulin in the absence of an infiltrative or granulomatous process |
| Splenomegaly |
| JAK2 V617F assay (peripheral blood; expression establishes the presence of an MPD but not its type; PV is always a consideration; absence does not exclude an MPD) |
| Increased circulating CD34+ cells (> 15 × 106/L) and no increase in marrow CD34+ cells by in situ immunohistochemistry |
| Characteristic cytogenetic abnormalities (peripheral blood: del(13q), 9p, del(20q), del(12p), partial trisomy1q, trisomy 8, and trisomy 9) |
| Absence of Bcr-Abl, AML, or MDS cytogenetic abnormalities by FISH (peripheral blood) |