CD150+ cells consistently include all or almost all of the HSC activity in adult bone marrow while CD150− cells include transiently reconstituting multipotent progenitors and little or no HSC activity
| Source, cells . | % of all cells . | Donor cell reconstitution, % of all recipient mice (n/N) . | HSC frequency . | % of all HSCs in this population . | ||
|---|---|---|---|---|---|---|
| Long-term multilineage . | Transient multilineage . | Other (e.g., lymphoid) . | ||||
| Adult bone marrow | ||||||
| 20,000 CD150+ | 8.8 | 93 (13/14) | 7.1 (1/14) | 0 (0/14) | 1 in 7,600 | 97.6 |
| 180,000 CD150− | 91 | 5.5 (1/18) | 89 (16/18) | 5.5 (1/18) | 1 in 3,150,000 | 2.4 |
| Adult bone marrow | ||||||
| 5 KS CD150+CD48− | 0.011 | 85 (11/13) | 0 (0/13) | 7.7 (1/13) | 1 in 3.2 | 95.2 |
| 5 KS CD150−CD48− | 0.024 | 3.6 (2/56) | 66 (37/56) | 20 (11/56) | 1 in 138.0 | 4.8 |
| 10 KS CD150−CD48− | 0.024 | 0 (0/7) | 57 (4/7) | 43 (3/7) | <1 in 70 | 0 |
| 5 KTLS CD150− | 0.005 | 0 (0/10) | 20 (2/10) | 20 (2/10) | <1 in 50 | 0 |
| Mobilized spleen | ||||||
| 20,000 CD150+ | 13 | 89 (8/9) | 11 (1/9) | 0 (0/9) | 1 in 9,100 | 100 |
| 180,000 CD150− | 87 | 0 (0/10) | 0 (0/10) | 100 (10/10) | <1 in 1,800,000 | 0 |
| Reconstituted bone marrow | ||||||
| 20,000 CD150+ | 12 | 75 (3/4) | 0 (0/4) | 0 (0/4) | 1 in 14,400 | 100 |
| 180,000 CD150− | 88 | 0 (0/5) | 0 (0/5) | 40 (2/5) | <1 in 900,000 | 0 |
| Source, cells . | % of all cells . | Donor cell reconstitution, % of all recipient mice (n/N) . | HSC frequency . | % of all HSCs in this population . | ||
|---|---|---|---|---|---|---|
| Long-term multilineage . | Transient multilineage . | Other (e.g., lymphoid) . | ||||
| Adult bone marrow | ||||||
| 20,000 CD150+ | 8.8 | 93 (13/14) | 7.1 (1/14) | 0 (0/14) | 1 in 7,600 | 97.6 |
| 180,000 CD150− | 91 | 5.5 (1/18) | 89 (16/18) | 5.5 (1/18) | 1 in 3,150,000 | 2.4 |
| Adult bone marrow | ||||||
| 5 KS CD150+CD48− | 0.011 | 85 (11/13) | 0 (0/13) | 7.7 (1/13) | 1 in 3.2 | 95.2 |
| 5 KS CD150−CD48− | 0.024 | 3.6 (2/56) | 66 (37/56) | 20 (11/56) | 1 in 138.0 | 4.8 |
| 10 KS CD150−CD48− | 0.024 | 0 (0/7) | 57 (4/7) | 43 (3/7) | <1 in 70 | 0 |
| 5 KTLS CD150− | 0.005 | 0 (0/10) | 20 (2/10) | 20 (2/10) | <1 in 50 | 0 |
| Mobilized spleen | ||||||
| 20,000 CD150+ | 13 | 89 (8/9) | 11 (1/9) | 0 (0/9) | 1 in 9,100 | 100 |
| 180,000 CD150− | 87 | 0 (0/10) | 0 (0/10) | 100 (10/10) | <1 in 1,800,000 | 0 |
| Reconstituted bone marrow | ||||||
| 20,000 CD150+ | 12 | 75 (3/4) | 0 (0/4) | 0 (0/4) | 1 in 14,400 | 100 |
| 180,000 CD150− | 88 | 0 (0/5) | 0 (0/5) | 40 (2/5) | <1 in 900,000 | 0 |
In rows where reconstitution categories do not add to 100%, the remaining mice were unreconstituted by donor cells. Some of these data were previously published.3,4 Transient multilineage reconstitution means myeloid chimerism was lost within 16 weeks of transplantation.
n indicates number in group; N, total number.