Cul4A deletion causes a dramatic loss of hematopoietic cells
| Genotype . | Weight, mg . | Total cellularity, ×106 . | Progenitors . |
|---|---|---|---|
| Bone marrow (femur) | |||
| Control | 11.62 ± 1.24 | 45 200 ± 5200 | |
| Cul4Aflox/flox Mx-Cre | 1.11 ± 0.27 | 12 ± 12 | |
| Spleen | |||
| Control | 87.2 ± 4.4 | 57.79 ± 7.6 | 9660 ± 2920 |
| Cul4Aflox/flox Mx-Cre | 45.6 ± 4.4 | 31.09 ± 8.1 | 121 ± 121 |
| Genotype . | Weight, mg . | Total cellularity, ×106 . | Progenitors . |
|---|---|---|---|
| Bone marrow (femur) | |||
| Control | 11.62 ± 1.24 | 45 200 ± 5200 | |
| Cul4Aflox/flox Mx-Cre | 1.11 ± 0.27 | 12 ± 12 | |
| Spleen | |||
| Control | 87.2 ± 4.4 | 57.79 ± 7.6 | 9660 ± 2920 |
| Cul4Aflox/flox Mx-Cre | 45.6 ± 4.4 | 31.09 ± 8.1 | 121 ± 121 |
Four days after induction of Cre with pIpC, Cul4Aflox/flox Mx-Cre mice and controls were killed (n=8 and n=7, respectively). Spleens were weighed, and the number of splenocytes per spleen was determined, as well as the total number of LDMNCs per femur. Splenocytes and LDMNCs were then cultured in progenitor cell methylcellulose colony assay, and after 7 days total colonies were counted, and the number of progenitors per spleen or femur was calculated. The results from 2 independent experiments were combined. Means and standard error of the mean are reported. P ≤ .03.