Percentages of cells positive on immunocytochemistry
. | Sham . | WT injured, d after injury (n = 15) . | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | ||||||||||
Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | |
TF | 16.7 | 3.5 | 75.3 | 5.6 | 84 | 5.0 | 85.3 | 4.9 | 78.7 | 6.2 | 57.3 | 7.5 | 34.3 | 8.2 | 23 | 6.9 |
PAR-1 | 8.3 | 1.8 | 80.3 | 9.8 | 86 | 3.8 | 87 | 4.2 | 85 | 4.6 | 78.3 | 2.4 | 64.3 | 14.5 | 46.7 | 11.9 |
PAR-2 | 8 | 1.5 | 75.7 | 8.1 | 81 | 5.3 | 85.7 | 7.1 | 87 | 4.6 | 76 | 3.6 | 51.3 | 5.8 | 33 | 4.6 |
PAR-4 | 7.3 | 1.2 | 62.7 | 7.6 | 70.3 | 6.2 | 71.3 | 8.7 | 72 | 9.3 | 69.3 | 9.2 | 59 | 8.7 | 38 | 10.1 |
α-SMA* | 2.3 | 0.9 | 3 | 1.2 | 8.3 | 1.5 | 13.7 | 1.5 | 14 | 2.1 | 12.7 | 2.4 | 10 | 0.6 | 9 | 3.1 |
FLK-1 | 15 | 2.1 | 75.7 | 6.7 | 85 | 4.6 | 89.7 | 3.3 | 87.7 | 4.9 | 68.7 | 6.1 | 52.7 | 6.1 | 42 | 7.7 |
CD31† | 8 | 2.6 | 40.7 | 6.1 | 56 | 5.5 | 59.3 | 4.9 | 57.7 | 7.8 | 41 | 4.1 | 32.7 | 4.6 | 16.3 | 3.5 |
Sca-1 | 6 | 2.1 | 3.3 | 1.2 | 1 | 0 | 0.7 | 0.3 | 0.7 | 0.3 | 1 | 0 | 2.7 | 1.2 | 3.3 | 1.5 |
CD133‡ | 1 | 0 | 0.7 | 0.3 | 0 | — | 0 | — | 0 | — | 0.7 | 0.3 | 0.7 | 0.3 | 1.33 | 0.3 |
. | Sham . | WT injured, d after injury (n = 15) . | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | ||||||||||
Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | Mean | SE | |
TF | 16.7 | 3.5 | 75.3 | 5.6 | 84 | 5.0 | 85.3 | 4.9 | 78.7 | 6.2 | 57.3 | 7.5 | 34.3 | 8.2 | 23 | 6.9 |
PAR-1 | 8.3 | 1.8 | 80.3 | 9.8 | 86 | 3.8 | 87 | 4.2 | 85 | 4.6 | 78.3 | 2.4 | 64.3 | 14.5 | 46.7 | 11.9 |
PAR-2 | 8 | 1.5 | 75.7 | 8.1 | 81 | 5.3 | 85.7 | 7.1 | 87 | 4.6 | 76 | 3.6 | 51.3 | 5.8 | 33 | 4.6 |
PAR-4 | 7.3 | 1.2 | 62.7 | 7.6 | 70.3 | 6.2 | 71.3 | 8.7 | 72 | 9.3 | 69.3 | 9.2 | 59 | 8.7 | 38 | 10.1 |
α-SMA* | 2.3 | 0.9 | 3 | 1.2 | 8.3 | 1.5 | 13.7 | 1.5 | 14 | 2.1 | 12.7 | 2.4 | 10 | 0.6 | 9 | 3.1 |
FLK-1 | 15 | 2.1 | 75.7 | 6.7 | 85 | 4.6 | 89.7 | 3.3 | 87.7 | 4.9 | 68.7 | 6.1 | 52.7 | 6.1 | 42 | 7.7 |
CD31† | 8 | 2.6 | 40.7 | 6.1 | 56 | 5.5 | 59.3 | 4.9 | 57.7 | 7.8 | 41 | 4.1 | 32.7 | 4.6 | 16.3 | 3.5 |
Sca-1 | 6 | 2.1 | 3.3 | 1.2 | 1 | 0 | 0.7 | 0.3 | 0.7 | 0.3 | 1 | 0 | 2.7 | 1.2 | 3.3 | 1.5 |
CD133‡ | 1 | 0 | 0.7 | 0.3 | 0 | — | 0 | — | 0 | — | 0.7 | 0.3 | 0.7 | 0.3 | 1.33 | 0.3 |