Factor VIII and VIIIa decay rates
| . | Factor VIII decay . | Factor VIIIa decay . | ||
|---|---|---|---|---|
| Thermostability at 55°C, min−1 . | Plasma stability, h−1 . | Factor IXa absent, min−1 . | Factor IXa present, min−1 . | |
| WT | 0.0471 (1.00) | 0.0178 (1.00) | 0.0836 (1.00) | 0.0154 (1.00) |
| E272A | 0.0542 (1.15) | ND | 0.1638 (1.95) | 0.0163 (1.06) |
| E272V | 0.0602 (1.28) | ND | 0.2271 (2.72) | 0.0159 (1.03) |
| D519A | 0.0336 (0.71)* | 0.0066 (0.37)* | 0.0556 (0.66)* | 0.0063 (0.41)* |
| D519V | 0.0262 (0.56)* | 0.0184 (1.03) | 0.0642 (0.77)* | 0.0068 (0.44)* |
| E665A | 0.0359 (0.76)* | 0.0149 (0.84)† | 0.0520 (0.62)* | 0.0078 (0.51)* |
| E665V | 0.0309 (0.66)* | 0.0047 (0.26)* | 0.0160 (0.19)* | 0.0052 (0.34)* |
| E1984A | 0.0240 (0.51)* | 0.0080 (0.45)* | 0.0241 (0.29)* | 0.0027 (0.18)* |
| E1984V | 0.0211 (0.45)* | 0.0078 (0.44)* | 0.0217 (0.26)* | 0.0019 (0.13)* |
| . | Factor VIII decay . | Factor VIIIa decay . | ||
|---|---|---|---|---|
| Thermostability at 55°C, min−1 . | Plasma stability, h−1 . | Factor IXa absent, min−1 . | Factor IXa present, min−1 . | |
| WT | 0.0471 (1.00) | 0.0178 (1.00) | 0.0836 (1.00) | 0.0154 (1.00) |
| E272A | 0.0542 (1.15) | ND | 0.1638 (1.95) | 0.0163 (1.06) |
| E272V | 0.0602 (1.28) | ND | 0.2271 (2.72) | 0.0159 (1.03) |
| D519A | 0.0336 (0.71)* | 0.0066 (0.37)* | 0.0556 (0.66)* | 0.0063 (0.41)* |
| D519V | 0.0262 (0.56)* | 0.0184 (1.03) | 0.0642 (0.77)* | 0.0068 (0.44)* |
| E665A | 0.0359 (0.76)* | 0.0149 (0.84)† | 0.0520 (0.62)* | 0.0078 (0.51)* |
| E665V | 0.0309 (0.66)* | 0.0047 (0.26)* | 0.0160 (0.19)* | 0.0052 (0.34)* |
| E1984A | 0.0240 (0.51)* | 0.0080 (0.45)* | 0.0241 (0.29)* | 0.0027 (0.18)* |
| E1984V | 0.0211 (0.45)* | 0.0078 (0.44)* | 0.0217 (0.26)* | 0.0019 (0.13)* |
Values are means. SDs for rate decay values are estimated based upon least squares curve fitting and are within approximately 10% of mean values for thermostability and factor VIIIa decay measurements and within approximately 15% of mean values for the plasma stability measurements. Values in parentheses are relative to the WT value. A single letter code is used to designate the amino acid residues, E (Glu), D (Asp), A (Ala), and V (Val).
ND indicates not determined.
P < .001 compared with the rate of WT (Student t test).
P < .05 compared with the rate of WT (Student t test).