Table 4 Engraftment data for... | American Society of Hematology

Table 4

Engraftment data for multilymphoid progenitor transplants assessed by FACS

Mouse ID	Vector	CID	Day post BMT	% huCD45⁺ cells	%CD34⁺Lin⁻/huCD45⁺ cells	Fold increase CD34⁺Lin⁻ human cells
Experiment A
1	F36V-MPL	+	49	46.7	13.8	154.7
2	F36V-MPL	+	49	19.9	20.8	114.1
3	F36V-MPL	−	49	0.14	0	0
4	F36V-MPL	−	49	0	0	0
5	F36V-MPL	−	49	0.49	0	0
6	Control (no transplant)		49	0	0	0
Experiment B
1	F36V-MPL	+	9	30.5	18.3	101
2	F36V-MPL	+	37	7.67	53.4	69
3	F36V-MPL	−	9	0.23	31.2	0.4
4	F36V-MPL	−	37	0.086	0	0
5	F36V-MPL	−	37	0.38	0	0
6	Control (no transplant)	−	37	0	0	0
Summary				Mean % huCD45⁺ cells	Mean %CD34⁺Lin⁻/huCD45⁺ cells	Mean fold increase CD34⁺Lin⁻ human cells
	F36V-MPL	+		26.2	26.5	109.7
	F36V-MPL	−		0.15	5.2	0.06

Mouse ID	Vector	CID	Day post BMT	% huCD45⁺ cells	%CD34⁺Lin⁻/huCD45⁺ cells	Fold increase CD34⁺Lin⁻ human cells
Experiment A
1	F36V-MPL	+	49	46.7	13.8	154.7
2	F36V-MPL	+	49	19.9	20.8	114.1
3	F36V-MPL	−	49	0.14	0	0
4	F36V-MPL	−	49	0	0	0
5	F36V-MPL	−	49	0.49	0	0
6	Control (no transplant)		49	0	0	0
Experiment B
1	F36V-MPL	+	9	30.5	18.3	101
2	F36V-MPL	+	37	7.67	53.4	69
3	F36V-MPL	−	9	0.23	31.2	0.4
4	F36V-MPL	−	37	0.086	0	0
5	F36V-MPL	−	37	0.38	0	0
6	Control (no transplant)	−	37	0	0	0
Summary				Mean % huCD45⁺ cells	Mean %CD34⁺Lin⁻/huCD45⁺ cells	Mean fold increase CD34⁺Lin⁻ human cells
	F36V-MPL	+		26.2	26.5	109.7
	F36V-MPL	−		0.15	5.2	0.06

Fold expansion of human CD34⁺Lin⁻ cells is derived from the percentage of huCD45⁺CD34⁺Lin⁻ cells and total cells harvested from bone marrow (femurs, tibias) at times shown divided by number of cells transplanted (n = 12 mice, 2 independent experiments), fold expansion of CD34⁺Lin⁻ cells is significantly greater in CID-treated mice (P = .006) compared with all other groups.

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