Hematopoietic phenotypes of partially trisomic mouse models
| Strain/fetal hematopoiesis . | Adult hematopoiesis . | Transplantability . | References . |
|---|---|---|---|
| Ts16 | |||
| Thymic hypoplasia, delayed maturation of T lymphocytes, decreased B lymphocytes, and stem cells deficiency | Not studied because Ts16 embryos die in utero | FL donor cells: decreased repopulating ability; anemia | 68,105 |
| Ts65Dn | |||
| No significant differences in in vitro colony formation or number of megakaryocytes compared with euploid littermates | Macrocytosis, decreased red cell number, thrombocytosis, BM fibrosis, extramedullary hematopoiesis, increased CD34+ cells (increased apoptosis in vitro), increased GMP, and less quiescent LSK cells | BM donor cells: weaker long-term repopulating ability; macrocytosis | 66,67 |
| Ts1Cje | |||
| Fewer and smaller colonies. Normal number of HSCs, but impaired CFU-S | Macrocytosis, decreased red cell number and late erythroid differentiated stages, increased GMP | FL donor cells: no competitive reconstitution; BM donor cells: macrocytosis and anemia | 71 |
| Strain/fetal hematopoiesis . | Adult hematopoiesis . | Transplantability . | References . |
|---|---|---|---|
| Ts16 | |||
| Thymic hypoplasia, delayed maturation of T lymphocytes, decreased B lymphocytes, and stem cells deficiency | Not studied because Ts16 embryos die in utero | FL donor cells: decreased repopulating ability; anemia | 68,105 |
| Ts65Dn | |||
| No significant differences in in vitro colony formation or number of megakaryocytes compared with euploid littermates | Macrocytosis, decreased red cell number, thrombocytosis, BM fibrosis, extramedullary hematopoiesis, increased CD34+ cells (increased apoptosis in vitro), increased GMP, and less quiescent LSK cells | BM donor cells: weaker long-term repopulating ability; macrocytosis | 66,67 |
| Ts1Cje | |||
| Fewer and smaller colonies. Normal number of HSCs, but impaired CFU-S | Macrocytosis, decreased red cell number and late erythroid differentiated stages, increased GMP | FL donor cells: no competitive reconstitution; BM donor cells: macrocytosis and anemia | 71 |
FL indicates fetal liver; BM, bone marrow; GMP, granulocyte macrophage progenitor; LSK, Lin−c-Kit+Sca1+; HSC, hematopoietic stem cell; and CFU-S, colony-forming unit–spleen.