Growth of childhood ALL cell population in suspension culture
Patient no. . | Karyotype . | Subfraction . | Percentage NC . | Absolute cell counts × 104 . | Percentage FISH+ (week 4/week 6) . | |
---|---|---|---|---|---|---|
Initiation . | Week 6 . | |||||
1 | t(12;21) | CD133+/CD19+ | 0.96 | 0.54 | 0 | — |
CD133+/CD19− | 0.48 | 0.72 | 23.8 | 100 | ||
2 | Hyperdiploid | CD133+/CD19+ | 72.26 | 100 | 0.1 | 75 |
CD133+/CD19− | 3.92 | 80.6 | 792.8 | 92 | ||
3 | del 9p, 17p | CD133+/CD19+ | 33.54 | 50 | 0.64 | 50 |
CD133+/CD19− | 0.21 | 1.7 | 140.8 | 62 | ||
4 | t(12;21) | CD133+/CD19+ | 0.55 | 1.8 | 0.96 | 50 |
CD133+/CD19− | 0.03 | 0.06 | 83.2 | 79 | ||
5 | t(12;21) | CD133+/CD19+ | 1.78 | 2.31 | 0.64 | * |
CD133+/CD19− | 0.09 | 0.4 | 454.4 | 76 | ||
6 | t(12;21) | CD133+/CD19+ | 0.42 | 1 | 0.13 | — |
CD133+/CD19− | 0.28 | 1 | 140.8 | 85 | ||
7 | t(12;17) | CD133+/CD19+ | 0.49 | 2.7 | 0 | — |
CD133+/CD19− | 0.06 | 0.05 | 131.2 | — | ||
8 | t(12;21) | CD133+/CD19+ | 3.81 | 3 | 6.4 | 100 |
CD133+/CD19− | 0.25 | 0.015 | 278.4 | 100 | ||
9 | Complex | CD133+/CD19+ | 1.92 | 9.32 | 0 | — |
CD133+/CD19− | 3.15 | 9.16 | 185.6 | 93 | ||
10 | +6 | CD133+/CD19+ | 0.33 | 0.22 | 1.14 | * |
CD133+/CD19− | 0.09 | 0.05 | 163.2 | 60 | ||
11 | 46XX | CD133+/CD19+ | 0.03 | 0.11 | 0 | — |
CD133+/CD19− | 0.20 | 0.37 | 768.3 | — | ||
12 | t(12;21) | CD133+/CD19+ | 0.17 | 0.22 | 0 | — |
CD133+/CD19− | 0.35 | 0.14 | 288 | 82 | ||
Median, CD133+/CD19+ | 0.76 | 2.06 | 0.12 | 62.5 | ||
Median, CD133+/CD19− | 0.23 | 0.39 | 174.4 | 83.5 |
Patient no. . | Karyotype . | Subfraction . | Percentage NC . | Absolute cell counts × 104 . | Percentage FISH+ (week 4/week 6) . | |
---|---|---|---|---|---|---|
Initiation . | Week 6 . | |||||
1 | t(12;21) | CD133+/CD19+ | 0.96 | 0.54 | 0 | — |
CD133+/CD19− | 0.48 | 0.72 | 23.8 | 100 | ||
2 | Hyperdiploid | CD133+/CD19+ | 72.26 | 100 | 0.1 | 75 |
CD133+/CD19− | 3.92 | 80.6 | 792.8 | 92 | ||
3 | del 9p, 17p | CD133+/CD19+ | 33.54 | 50 | 0.64 | 50 |
CD133+/CD19− | 0.21 | 1.7 | 140.8 | 62 | ||
4 | t(12;21) | CD133+/CD19+ | 0.55 | 1.8 | 0.96 | 50 |
CD133+/CD19− | 0.03 | 0.06 | 83.2 | 79 | ||
5 | t(12;21) | CD133+/CD19+ | 1.78 | 2.31 | 0.64 | * |
CD133+/CD19− | 0.09 | 0.4 | 454.4 | 76 | ||
6 | t(12;21) | CD133+/CD19+ | 0.42 | 1 | 0.13 | — |
CD133+/CD19− | 0.28 | 1 | 140.8 | 85 | ||
7 | t(12;17) | CD133+/CD19+ | 0.49 | 2.7 | 0 | — |
CD133+/CD19− | 0.06 | 0.05 | 131.2 | — | ||
8 | t(12;21) | CD133+/CD19+ | 3.81 | 3 | 6.4 | 100 |
CD133+/CD19− | 0.25 | 0.015 | 278.4 | 100 | ||
9 | Complex | CD133+/CD19+ | 1.92 | 9.32 | 0 | — |
CD133+/CD19− | 3.15 | 9.16 | 185.6 | 93 | ||
10 | +6 | CD133+/CD19+ | 0.33 | 0.22 | 1.14 | * |
CD133+/CD19− | 0.09 | 0.05 | 163.2 | 60 | ||
11 | 46XX | CD133+/CD19+ | 0.03 | 0.11 | 0 | — |
CD133+/CD19− | 0.20 | 0.37 | 768.3 | — | ||
12 | t(12;21) | CD133+/CD19+ | 0.17 | 0.22 | 0 | — |
CD133+/CD19− | 0.35 | 0.14 | 288 | 82 | ||
Median, CD133+/CD19+ | 0.76 | 2.06 | 0.12 | 62.5 | ||
Median, CD133+/CD19− | 0.23 | 0.39 | 174.4 | 83.5 |
NC indicates nucleated cells; and —, not applicable.
FISH probe hybridization unsuccessful.