Table 3 Immunophenotypic and karyotypic... | American Society of Hematology

Table 3

Immunophenotypic and karyotypic analyses of BM cells from serial xenografts

Patient no.	Xenograft	No. of cells injected	% CD45⁺ in BM	% FISH⁺	Immunophenotype*
1	Primary	6.5 × 10³	20 ± 4	100	CD3⁻/CD10⁺/CD19⁺/CD33⁻/ CD34⁺
	Secondary	6.5 × 10³	25 ± 3	100	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
5	Primary	1.8 × 10³	5	82	CD3⁻/CD10⁺/CD19⁺/CD34⁺
	Secondary	1.8 × 10³	7 ± 3	76	CD3⁻/CD10⁺/CD19⁺/CD34⁺
6	Primary	5.4 × 10³	18 ± 6	85	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
	Secondary	5.4 × 10³	15 ± 5	72	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
8	Primary	7.9 × 10³	8 ± 2	90	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
	Secondary	7.9 × 10³	11 ± 3	85	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
14	Primary	5.4 × 10³	35 ± 6	—	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
	Secondary	5.4 × 10³	30 ± 7	—	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺

Patient no.	Xenograft	No. of cells injected	% CD45⁺ in BM	% FISH⁺	Immunophenotype*
1	Primary	6.5 × 10³	20 ± 4	100	CD3⁻/CD10⁺/CD19⁺/CD33⁻/ CD34⁺
	Secondary	6.5 × 10³	25 ± 3	100	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
5	Primary	1.8 × 10³	5	82	CD3⁻/CD10⁺/CD19⁺/CD34⁺
	Secondary	1.8 × 10³	7 ± 3	76	CD3⁻/CD10⁺/CD19⁺/CD34⁺
6	Primary	5.4 × 10³	18 ± 6	85	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
	Secondary	5.4 × 10³	15 ± 5	72	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
8	Primary	7.9 × 10³	8 ± 2	90	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
	Secondary	7.9 × 10³	11 ± 3	85	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
14	Primary	5.4 × 10³	35 ± 6	—	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺
	Secondary	5.4 × 10³	30 ± 7	—	CD3⁻/CD10⁺/CD19⁺/CD33⁻/CD34⁺

Primary indicates primary NOD/SCID recipient; Secondary, secondary NOD/SCID recipient; and —, not applicable.

The cutoff point for positivity was set at 20% of cells staining more intensely than the isotype control, in accordance with standard diagnostic immunophenotyping procedures defined by the British Committee for Standards in Haematology.³¹

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