Table 2 Functional profiles of... | American Society of Hematology

Table 2

Functional profiles of HHV-8–specific CD8⁺ T cells in MCD patients and AC

Patient	Peptide stimulation	Functions of HHV-8–specific CD8⁺ T cells*						HHV-8 viral load (copies/1.5 10⁵ PBMC)	CD4 cell count/mm³
Patient	Peptide stimulation	IFN-γ⁺	IL-2⁺	TNF-α⁺	CD107a⁺	MIP1β⁺	Number of functions†	HHV-8 viral load (copies/1.5 10⁵ PBMC)	CD4 cell count/mm³
MCD REP	None	0.01	0.12	0.01	0.16	0.09
	K15 pools, GpH_59-68, GpB_492-500	0.21	0.10	0.03	0.15	0.12
	Δ	0.20	0.00	0.02	0.00	0.00	1	837	305
MCD NEG	None	0.15	0.06	0.03	0.16	0.56
	GpH_59-68, GpB_492-500	1.54	0.09	0.18	0.21	1.30
	Δ	1.39	0.03	0.15	0.05	0.74	3	240	34
MCD UAG	None	0.02	0.13	0.02	0.40	0.53
	K12_31-45, K15_166-180	0.50	0.10	0.43	0.84	1.23
	Δ	0.48	0.00	0.43	0.44	0.70	4	12 770	1789
AC 210	None	0.06	0.28	0.02	0.99	0.21
	K12_31-45, GpH_59-68, GpB_492-500	0.44	0.21	0.06	1.07	0.90
	Δ	0.38	0.00	0.04	0.08	0.69	2	<10	279
AC 12001	None	0.01	0.03	0.07	0.13	0.26
	K12_31-45, LANA_1116-1124	0.19	0.08	0.11	0.45	0.78
	Δ	0.18	0.05	0.04	0.32	0.52	3	<10	488
AC 11007	None	0.12	0.19	0.11	0.80	1.28
	GpH_59-68, GpB_492-500	1.45	0.17	0.86	1.63	3.44
	Δ	1.33	0.00	0.75	0.83	2.16	4	<10	709
AC NAH	None	0.06	0.12	0.06	0.43	0.76
	GpH_59-68, GpB_492-500	0.44	0.17	0.33	0.95	1.67
	Δ	0.38	0.05	0.27	0.52	0.91	4	<10	361

Patient	Peptide stimulation	Functions of HHV-8–specific CD8⁺ T cells*						HHV-8 viral load (copies/1.5 10⁵ PBMC)	CD4 cell count/mm³
Patient	Peptide stimulation	IFN-γ⁺	IL-2⁺	TNF-α⁺	CD107a⁺	MIP1β⁺	Number of functions†	HHV-8 viral load (copies/1.5 10⁵ PBMC)	CD4 cell count/mm³
MCD REP	None	0.01	0.12	0.01	0.16	0.09
	K15 pools, GpH_59-68, GpB_492-500	0.21	0.10	0.03	0.15	0.12
	Δ	0.20	0.00	0.02	0.00	0.00	1	837	305
MCD NEG	None	0.15	0.06	0.03	0.16	0.56
	GpH_59-68, GpB_492-500	1.54	0.09	0.18	0.21	1.30
	Δ	1.39	0.03	0.15	0.05	0.74	3	240	34
MCD UAG	None	0.02	0.13	0.02	0.40	0.53
	K12_31-45, K15_166-180	0.50	0.10	0.43	0.84	1.23
	Δ	0.48	0.00	0.43	0.44	0.70	4	12 770	1789
AC 210	None	0.06	0.28	0.02	0.99	0.21
	K12_31-45, GpH_59-68, GpB_492-500	0.44	0.21	0.06	1.07	0.90
	Δ	0.38	0.00	0.04	0.08	0.69	2	<10	279
AC 12001	None	0.01	0.03	0.07	0.13	0.26
	K12_31-45, LANA_1116-1124	0.19	0.08	0.11	0.45	0.78
	Δ	0.18	0.05	0.04	0.32	0.52	3	<10	488
AC 11007	None	0.12	0.19	0.11	0.80	1.28
	GpH_59-68, GpB_492-500	1.45	0.17	0.86	1.63	3.44
	Δ	1.33	0.00	0.75	0.83	2.16	4	<10	709
AC NAH	None	0.06	0.12	0.06	0.43	0.76
	GpH_59-68, GpB_492-500	0.44	0.17	0.33	0.95	1.67
	Δ	0.38	0.05	0.27	0.52	0.91	4	<10	361

Number of CD8⁺ cells producing IFN-γ, IL-2, TNF-α, CD107a, or MIP1β/number of CD8⁺ cells, as assessed by intracellular staining.

Positive threshold: >0.1% of CD8⁺ cells, >twice background values, and >200 absolute number of CD8⁺ cytokine/chemokine⁺ cells.