The mutation frequency of heavy chain rearrangements from IgD+CD19+ B cells of individual patients with X-HIgM of different ages
| . | 4-year-old X-HIgM . | 9-year-old X-HIgM . | 7- to 12-year-old X-HIgM . | Adult X-HIgM . | ND adult . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| NP . | P . | NP . | P . | NP . | P . | NP . | P . | NP . | P . | |
| Total no. of rearrangements | 23 | 92 | 31 | 143 | 104 | 509 | 51 | 182 | 13 | 69 |
| Percentage of mutated rearrangements (%) | 6/23 (26)* | 14/92 (15) | 3/331 (1) | 11/143 (8) | 10/104 (10) | 38/509 (8) | 2/51 (4) | 17/182 (9) | 11/13 (85)* | 53/69 (77) |
| Total no. of mutations | 10 | 18 | 3 | 11 | 14 | 103 | 11 | 48 | 126 | 440 |
| Overall mutation frequency (%) | 10/5814 (0.2)† | 18/23208 (0.08) | 3/6540 (0.05) | 11/31315 (0.04) | 14/22461 (0.06) | 103/100669 (0.1) | 11/11345 (0.1) | 48/40687 (0.2) | 126/3330 (3.8)† | 440/17579 (2.5) |
| Mutation frequency of mutated rearrangements (%) | 10/1524 (0.7) | 18/3540 (0.6) | 3/644 (0.5) | 11/2377 (0.5) | 14/2022 (0.7) | 103/7548 (1.4) | 11/465 (2.4)‡ | 48/4126 (1.2) | 126/2826 (4.5)‡ | 440/13484 (3.3) |
| . | 4-year-old X-HIgM . | 9-year-old X-HIgM . | 7- to 12-year-old X-HIgM . | Adult X-HIgM . | ND adult . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| NP . | P . | NP . | P . | NP . | P . | NP . | P . | NP . | P . | |
| Total no. of rearrangements | 23 | 92 | 31 | 143 | 104 | 509 | 51 | 182 | 13 | 69 |
| Percentage of mutated rearrangements (%) | 6/23 (26)* | 14/92 (15) | 3/331 (1) | 11/143 (8) | 10/104 (10) | 38/509 (8) | 2/51 (4) | 17/182 (9) | 11/13 (85)* | 53/69 (77) |
| Total no. of mutations | 10 | 18 | 3 | 11 | 14 | 103 | 11 | 48 | 126 | 440 |
| Overall mutation frequency (%) | 10/5814 (0.2)† | 18/23208 (0.08) | 3/6540 (0.05) | 11/31315 (0.04) | 14/22461 (0.06) | 103/100669 (0.1) | 11/11345 (0.1) | 48/40687 (0.2) | 126/3330 (3.8)† | 440/17579 (2.5) |
| Mutation frequency of mutated rearrangements (%) | 10/1524 (0.7) | 18/3540 (0.6) | 3/644 (0.5) | 11/2377 (0.5) | 14/2022 (0.7) | 103/7548 (1.4) | 11/465 (2.4)‡ | 48/4126 (1.2) | 126/2826 (4.5)‡ | 440/13484 (3.3) |
The data for the 4-year-old and 9-year-old are from the same patient after a 5-year interval. The data from 3 different patients were combined in each of the 7- to 12-year-old and adult X-HIgM categories. The normal donor data include GenBank accession nos. X87006-87089 (excluding X87017 and X87083). The mutation frequencies were calculated from the number of mutations/total number of bases analyzed and from the total number of bases analyzed from mutated sequences only.
ND indicates normal donor; NP, nonproductive rearrangement; and P, productive rearrangement.
Significant (P ≤ .03) difference in the percentage of mutated NP rearrangements from 4-year-old X-HIgM versus other X-HIgM and ND versus all X-HIgM.
Significant (P < .05) difference in the overall mutation frequency of NP rearrangements from 4-year-old X-HIgM versus all other X-HIgM and ND versus all X-HIgM.
Significant (P < .005) difference in the mutation frequency of mutated NP rearrangements from ND versus all 4- to 12-year-old X-HIgM and adult X-HIgM versus other X-HIgM.