Frequency and distribution of individual nucleotide mutations
| . | X-HIgM . | Normal donors . | ||
|---|---|---|---|---|
| NP . | P . | NP . | P . | |
| RGYW (%) | 8.5/51 (17) | 56.5/250 (23)* | 147/593 (25)* | 1227.3/4857 (25)* |
| WRCY (%) | 9.5/51 (19) | 31.5/250 (13) | 84/593 (14) | 697.5/4857 (14) |
| WA (%) | 8/51 (16)† | 54/250 (22)‡ | 137.4/5933 (23)‡ | 890.5/4857 (18)‡ |
| TW | 6/51 (12) | 22/250 (9) | 66.7/593 (11) | 488.5/4857 (10) |
| FR (R/S) | 21/12 (1.8) | 114/50 (2.3) | 304/92 (3.3)§ | 1961/1155 (1.7) |
| CDR (R/S) | 14/4 (3.5) | 68/20 (3.4) | 166/29 (5.7)‖ | 1347/394 (3.4) |
| Transitions (%) | 33/51 (65)¶ | 140/250 (56) | 302/593 (51) | 2626/4857 (54) |
| Transversions (%) | 18/51 (35) | 110/250 (44) | 291/593 (49) | 2231/4857 (46) |
| . | X-HIgM . | Normal donors . | ||
|---|---|---|---|---|
| NP . | P . | NP . | P . | |
| RGYW (%) | 8.5/51 (17) | 56.5/250 (23)* | 147/593 (25)* | 1227.3/4857 (25)* |
| WRCY (%) | 9.5/51 (19) | 31.5/250 (13) | 84/593 (14) | 697.5/4857 (14) |
| WA (%) | 8/51 (16)† | 54/250 (22)‡ | 137.4/5933 (23)‡ | 890.5/4857 (18)‡ |
| TW | 6/51 (12) | 22/250 (9) | 66.7/593 (11) | 488.5/4857 (10) |
| FR (R/S) | 21/12 (1.8) | 114/50 (2.3) | 304/92 (3.3)§ | 1961/1155 (1.7) |
| CDR (R/S) | 14/4 (3.5) | 68/20 (3.4) | 166/29 (5.7)‖ | 1347/394 (3.4) |
| Transitions (%) | 33/51 (65)¶ | 140/250 (56) | 302/593 (51) | 2626/4857 (54) |
| Transversions (%) | 18/51 (35) | 110/250 (44) | 291/593 (49) | 2231/4857 (46) |
The X-HIgM data include 104 productive (P) and 24 nonproductive (NP) CD19+IgD+ and CD19+CD27+ mutated rearrangements. Normal donor populations include 483 P and 69 NP CD19+IgM+, IgD+CD27+, and IgD−CD27+ mutated rearrangements.
FR indicates framework region; CDR, complementarity determining region; R/S indicates replacement-to-silent mutation ratio. All positions of the motif were included for mutation analysis. AGCT motifs with a mutated G but not C were considered RGYW motif mutations. AGCT motifs with a mutated C but not G were considered WRCY motif mutations. Three ambiguous A or T mutations in AGCT motifs with neither G or C mutations were scored as a mutation in RGYW and WRCY motifs.
Significant (P < .05) difference between RGYW and WRCY mutations.
Significant (P = .03) difference between X-HIgM and normal donors nonproductive rearrangements.
Significantly (P = .05) greater mutation frequency compared with the expected random frequency.
Significant (P < .05) difference between the NP and P FR R/S of ND.
Significant (P = .02) difference between the NP FR R:S and NP CDR R:S of ND.
Significant (P = .03) difference between the percentage of transitions versus transversions.