CpG ODNs promote donor BM graft rejection independent of host IFNγ, IL-12, and IL-6
| Donor . | Recipient . | TBI, Gy . | CpG . | Survival . | Engraftment rate . | Donor chimerism, mean % ± 1 SEM . |
|---|---|---|---|---|---|---|
| BALB/c | B6 | 6.0 | No | 29/33 | 25/29 | 70 ± 6 |
| BALB/c | B6 | 6.0 | Yes | 18/33* | 9/18* | 31 ± 8* |
| B6 Ly5.2 | bm12 | 5.5 | No | 14/15 | 14/14 | 77 ± 6 |
| B6 Ly5.2 | bm12 | 5.5 | Yes | 15/15 | 15/15 | 39 ± 5* |
| B6 Ly5.2 | bm1 | 5.5 | No | 15/15 | 15/15 | 91 ± 2 |
| B6 Ly5.2 | bm1 | 5.5 | Yes | 14/15 | 8/14* | 37 ± 11* |
| BALB/c | B6 IFNγ−/− | 6.0 | No | 12/12 | 11/12 | 64 ± 9 |
| BALB/c | B6 IFNγ−/− | 6.0 | Yes | 9/12 | 1/9* | 9 ± 9* |
| BALB/c | B6 IL-12−/− | 6.0 | No | 10/12 | 6/10 | 44 ± 15 |
| BALB/c | B6 IL-12−/− | 6.0 | Yes | 5/12* | 0/5* | 0 ± 0* |
| BALB/c | B6 IL-6−/− | 6.0 | No | 12/12 | 11/12 | 79 ± 11 |
| BALB/c | B6 IL-6−/− | 6.0 | Yes | 6/12* | 3/6* | 14 ± 10* |
| Donor . | Recipient . | TBI, Gy . | CpG . | Survival . | Engraftment rate . | Donor chimerism, mean % ± 1 SEM . |
|---|---|---|---|---|---|---|
| BALB/c | B6 | 6.0 | No | 29/33 | 25/29 | 70 ± 6 |
| BALB/c | B6 | 6.0 | Yes | 18/33* | 9/18* | 31 ± 8* |
| B6 Ly5.2 | bm12 | 5.5 | No | 14/15 | 14/14 | 77 ± 6 |
| B6 Ly5.2 | bm12 | 5.5 | Yes | 15/15 | 15/15 | 39 ± 5* |
| B6 Ly5.2 | bm1 | 5.5 | No | 15/15 | 15/15 | 91 ± 2 |
| B6 Ly5.2 | bm1 | 5.5 | Yes | 14/15 | 8/14* | 37 ± 11* |
| BALB/c | B6 IFNγ−/− | 6.0 | No | 12/12 | 11/12 | 64 ± 9 |
| BALB/c | B6 IFNγ−/− | 6.0 | Yes | 9/12 | 1/9* | 9 ± 9* |
| BALB/c | B6 IL-12−/− | 6.0 | No | 10/12 | 6/10 | 44 ± 15 |
| BALB/c | B6 IL-12−/− | 6.0 | Yes | 5/12* | 0/5* | 0 ± 0* |
| BALB/c | B6 IL-6−/− | 6.0 | No | 12/12 | 11/12 | 79 ± 11 |
| BALB/c | B6 IL-6−/− | 6.0 | Yes | 6/12* | 3/6* | 14 ± 10* |
Indicated recipient mice were irradiated with 5.5 or 6.0 Gy TBI on d−1 and infused with 10 × 106 TCD donor BM cells from indicated strain on d0. CpG ODNs were given intraperitoneally on d0 and d7 (100 μg/dose). Survival indicates proportion of mice that received donor BM that survived to PBL phenotyping at approximately d35 after BMT. Engraftment rate indicates proportion of phenotyped mice that had a minimum of 5% donor chimerism. The effect of CpG ODNs on the different recipient strains was evaluated in separate experiments.
P < .05 compared with relevant untreated control.