Table 1 List of antibodies and... | American Society of Hematology

Table 1

List of antibodies and combinations used to identify B-cell subsets

Lymphocyte subsets	Distinguishing antibody combinations
Total T cells	CD4⁺CD19⁻
Total B cells	CD19⁺
Naive B cells	CD19⁺IgD⁺CD38⁻CD27⁻
Pro-GC B cells	CD19⁺IgD⁺CD38⁻IgM⁺CD23⁻FSC^HI
Pre-GC B cells	CD19⁺IgD⁺CD38⁺IgM⁺CD27⁻
GC B cells	CD19⁺IgD⁻CD38⁺
Plasmablast B cells	CD19⁺IgD⁻CD38⁺⁺CD27⁺
Memory B cells	CD19⁺IgD⁻CD38⁻CD27⁺

Lymphocyte subsets	Distinguishing antibody combinations
Total T cells	CD4⁺CD19⁻
Total B cells	CD19⁺
Naive B cells	CD19⁺IgD⁺CD38⁻CD27⁻
Pro-GC B cells	CD19⁺IgD⁺CD38⁻IgM⁺CD23⁻FSC^HI
Pre-GC B cells	CD19⁺IgD⁺CD38⁺IgM⁺CD27⁻
GC B cells	CD19⁺IgD⁻CD38⁺
Plasmablast B cells	CD19⁺IgD⁻CD38⁺⁺CD27⁺
Memory B cells	CD19⁺IgD⁻CD38⁻CD27⁺

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