Effects of DR and age on HSC frequencies using the mKSL criterion
| Donor type . | LT-HSC . | ST-HSC . | MPP . |
|---|---|---|---|
| BALB | |||
| Young AL | 99 ± 19 | 24 ± 5 | 1.9 ± 0.8 |
| Old AL | 1847 ± 341* | 251 ± 112† | 112.7 ± 80.8† |
| Old DR | 752 ± 145* | 87 ± 5‡ | 23.1 ± 4.8‡ |
| B6 | |||
| Young AL | 373 ± 26 | 852 ± 147 | 517 ± 146 |
| Old AL | 991 ± 122* | 1242 ± 341 | 787 ± 294 |
| Old DR | 1317 ± 117* | 810 ± 197 | 472 ± 135 |
| Donor type . | LT-HSC . | ST-HSC . | MPP . |
|---|---|---|---|
| BALB | |||
| Young AL | 99 ± 19 | 24 ± 5 | 1.9 ± 0.8 |
| Old AL | 1847 ± 341* | 251 ± 112† | 112.7 ± 80.8† |
| Old DR | 752 ± 145* | 87 ± 5‡ | 23.1 ± 4.8‡ |
| B6 | |||
| Young AL | 373 ± 26 | 852 ± 147 | 517 ± 146 |
| Old AL | 991 ± 122* | 1242 ± 341 | 787 ± 294 |
| Old DR | 1317 ± 117* | 810 ± 197 | 472 ± 135 |
BMCs from a total of 3–8 female BALB or B6 mice per group (Young AL, Young DR, Old AL, and Old DR) were analyzed by FACS before intravenous injection into lethally irradiated recipients. LT-HSCs, ST-HSCs, and MPPs present in the original donor marrow are given as number per 106 cells ± SE. After gating for lin−, cKit+, Sca1+, these BMCs were identified as LT-HSCs (CD34−, Flk2−), ST-HSCs (CD34+, Flk2−) and MPPs (CD34+, Flk2+).
P < .001 versus young AL controls.
P < .01 versus young AL controls.
P < .05 versus young AL controls.