Absence of Pten expression in mouse T-ALL cell lines does not predict GSI resistance
| Cell line . | Genotype . | Pten expression . | p-Akt detected . | p-p70 S6 k detected . | Notch status . | ICN detected . | GSI . |
|---|---|---|---|---|---|---|---|
| 130 | Tal1/HEB+/− | + | – | + | PEST insertion | Truncated | Sensitive |
| 135 | Tal1/HEB+/− | – | + | + | PEST insertion | Truncated | Sensitive |
| 720 | Tal1/HEB+/− | + | – | + | PEST insertion (2361) | Truncated | Sensitive |
| 2869 | Tal1/Ink4a/Arf+/− | + | – | + | No mutation | Full length | Sensitive |
| 2912 | Tal1/Ink4a/Arf+/− | + | – | + | No mutation | ND | Sensitive |
| 2913 | Tal1/Ink4a/Arf+/− | + | – | + | No mutation | ND | Sensitive |
| 3569 | Tal1/Ink4a/Arf+/− | – | + | + | HD mutation (L1699P) | Full length | Sensitive |
| 5010 | Tal1/+ | – | + | + | No mutation | Full length | Resistant |
| 5046 | Tal1/+ | – | + | + | PEST Insertion (2420) | Truncated | Sensitive |
| 5151 | Tal1/+ | – | + | + | PEST deletion (2492) | Truncated | Sensitive |
| Cell line . | Genotype . | Pten expression . | p-Akt detected . | p-p70 S6 k detected . | Notch status . | ICN detected . | GSI . |
|---|---|---|---|---|---|---|---|
| 130 | Tal1/HEB+/− | + | – | + | PEST insertion | Truncated | Sensitive |
| 135 | Tal1/HEB+/− | – | + | + | PEST insertion | Truncated | Sensitive |
| 720 | Tal1/HEB+/− | + | – | + | PEST insertion (2361) | Truncated | Sensitive |
| 2869 | Tal1/Ink4a/Arf+/− | + | – | + | No mutation | Full length | Sensitive |
| 2912 | Tal1/Ink4a/Arf+/− | + | – | + | No mutation | ND | Sensitive |
| 2913 | Tal1/Ink4a/Arf+/− | + | – | + | No mutation | ND | Sensitive |
| 3569 | Tal1/Ink4a/Arf+/− | – | + | + | HD mutation (L1699P) | Full length | Sensitive |
| 5010 | Tal1/+ | – | + | + | No mutation | Full length | Resistant |
| 5046 | Tal1/+ | – | + | + | PEST Insertion (2420) | Truncated | Sensitive |
| 5151 | Tal1/+ | – | + | + | PEST deletion (2492) | Truncated | Sensitive |
GSI indicates γ-secretase inhibitors; and ND, not detected.