List of primers used to assess transcript levels in murine HSCs and various downstream cell types
| Gene . | GenBank no. . | Forward primer . | Reverse primer . |
|---|---|---|---|
| Gapdh | NM_008084 | 5′-AACTTTGGCATTGTGGAAGG-3′ | 5′-ATGCAGGGATGATGTTCTGG-3′ |
| Cdkn2c | NM_007671 | 5′-GAACTGCGCTGCAGGTTAT-3′ | 5′-TCAAATTGGGATTAGCACCTC-3′ |
| Stat3 | NM_213659 | 5′-GGCACCTTGGATTGAGAGTC-3′ | 5′-ACTCTTGCAGGAATCGGCTA-3′ |
| MEF | NM_019680 | 5′-TCTGTGGATGAGGAGGTTCC-3′ | 5′-GGGTGCTGGAGAAGAACTCA-3′ |
| ATM | NM_007499 | 5′-GCAGAGTGTCTGAGGGTTTGT-3′ | 5′-AACTTCCAGCAACCTTCACC-3′ |
| Gata2 | NM_008090 | 5′-GCAGAGTGTCTGAGGGTTTGT-3′ | 5′-AACTTCCAGCAACCTTCACC-3′ |
| Lyl1 | NM_008535.2 | 5′-GACCCTTCAGCATCTTCCCTAACA-3′ | 5′-AGCCACCTTCTGGGGTTGGT-3′ |
| G3bp1 | NM_013716.2 | 5′-GGGAAGCCAGCAGATGCAGT-3′ | 5′-ATCCACGTGGCGGATCTTG-3′ |
| Eif1a | NM_010120.3 | 5′-CCGCTGCGTTTTGGTCACTA-3′ | 5′-TCGGTTCTGGCCTGGTTCTC-3′ |
| Tubb5 | NM_011655.3 | 5′-CAGCTGGACCGAATCTCTGTGT-3′ | 5′-GGACCTGAGCGAACGGAGTC-3′ |
| Hmga2 | NM_010441.1 | 5′-GGTGCCACAGAAGCGAGGAC-3′ | 5′-GGGCTCACAGGTTGGCTCTT-3′ |
| Psap | NM_011179.2 | 5′-ACTGTGGGGCCGTGAAGC-3′ | 5′-GTCGCAAGGAAGGGATTTCG-3′ |
| Prnp | NM_011170.1 | 5′-TCCAATTTAGGAGAGCCAAGCA-3′ | 5′-GCCGACATCAGTCCACATAGTCA-3′ |
| Pld3 | NM_0111161.1 | 5′-GCTGAGGAACCGGAAGCTGT-3′ | 5′-GGGAAAGGGGTGGTCCTGAG-3′ |
| Rhob | NM_007483.2 | 5′-GGGGCACGCAGAGTGGTT-3′ | 5′-GCAACAGTAGTGGCTTGCTGGTT-3′ |
| Vwf | NM_011708.3 | 5′-GGCGAGGATGGAGTTCGACA-3′ | 5′-TGACAGGGCTGATGGTCTGG-3′ |
| Car3 | NM_007606.3 | 5′-TGGCTAAGGAGTGGGGCTAC-3′ | 5′-GTCCCCTTTGGCAATTGGAT-3′ |
| Plp1 | NM_011123.2 | 5′-TCAGTCTATTGCCTTCCCTAGCAA-3′ | 5′-GCATTCCATGGGAGAACACCA-3′ |
| Hdac3 | NM_010411 | 5′-TCAACGTGGGTGATGACTGC-3′ | 5′-GCAGAGATGCGCCTGTGTA-3′ |
| Chd4 | NM_145979 | 5′-GCTGCCAGAGATCCCAAACG-3′ | 5′-TTGCCCTTAAGAGCTGGACAA-3′ |
| Cul4a | NM_146207 | 5′-GGAGAACATTTGACAGCAATTCTACA-3′ | 5′-GTGAGGTCGGGCACCCTGT-3′ |
| Trim27 | NM_009054 | 5′-GAAGAGACGGCGGGCACA-3′ | 5′-GCTGCTCAAACTCCCAGACAA-3′ |
| Fus | NM_139149 | 5′-CACTGGTTGCATTCATTTCTCCA-3′ | 5′-CCAGTGGAGGTGGTGGAGGT-3′ |
| Cebpa | NM_007678 | 5′-CCAGTCAGACCAGAAAGCTGA-3′ | 5′-CCACAAAGCCCAGAAACCTA-3′ |
| Mlx | NM_011550 | 5′-TGTGTCTTCAGCTGGATTGAGGA-3′ | 5′-GGACACCGATCACAATCTCTCG-3′ |
| Smarcc1 | NM_009211 | 5′-TGGGAGAGCCCGGACACG-3′ | 5′-TTGGTAGGAGCATCTGCATGAAC-3′ |
| Smarcc2 | NM_198160 | 5′-TCTTCAGCCGAAGCCTCCAC-3′ | 5′-CCCTTCTCAGGGAAGTTCAGCA-3′ |
| Cdkn1a | NM_007669 | 5′-GTACTTCCTCTGCCCTGCTG-3′ | 5′-TCTGCGCTTGGAGTGATAGA-3′ |
| 1200009F10Rik | NM_026166 | 5′-GAAAAACAATACCGGTTACTGCAAA-3′ | 5′-CCACGAGAGCTTCACATTCCTG-3′ |
| Ezh2 | NM_007971.1 | 5′-CGCTCTTCTGTCGACGATGTTTT-3′ | 5′-GTTGGGTGTTGCATGGAAGG-3′ |
| Gata3 | NM_008091.2 | 5′-GGTATCCTCCGACCCACCAC-3′ | 5′-CCAGCCAGGGCAGAGATCC-3′ |
| Bmi1 | NM_007552.3 | 5′-AAACCAGACCACTCCTGAACA-3′ | 5′-TCTTCTTCTCTTCATCTCATTTTTGA-3′ |
| Sh2b3 | NM_008507 | 5′-CAACACACACAAGGCTGTCA-3′ | 5′-CCTGTGCACAAGAACTACATCTG-3′ |
| Rae28 | NM_001042623 | 5′-CGCACATCATTGAAGGCTTTGTT-3′ | 5′-TTCTTTCAGGAACTGAGAACATCC-3′ |
| Gene . | GenBank no. . | Forward primer . | Reverse primer . |
|---|---|---|---|
| Gapdh | NM_008084 | 5′-AACTTTGGCATTGTGGAAGG-3′ | 5′-ATGCAGGGATGATGTTCTGG-3′ |
| Cdkn2c | NM_007671 | 5′-GAACTGCGCTGCAGGTTAT-3′ | 5′-TCAAATTGGGATTAGCACCTC-3′ |
| Stat3 | NM_213659 | 5′-GGCACCTTGGATTGAGAGTC-3′ | 5′-ACTCTTGCAGGAATCGGCTA-3′ |
| MEF | NM_019680 | 5′-TCTGTGGATGAGGAGGTTCC-3′ | 5′-GGGTGCTGGAGAAGAACTCA-3′ |
| ATM | NM_007499 | 5′-GCAGAGTGTCTGAGGGTTTGT-3′ | 5′-AACTTCCAGCAACCTTCACC-3′ |
| Gata2 | NM_008090 | 5′-GCAGAGTGTCTGAGGGTTTGT-3′ | 5′-AACTTCCAGCAACCTTCACC-3′ |
| Lyl1 | NM_008535.2 | 5′-GACCCTTCAGCATCTTCCCTAACA-3′ | 5′-AGCCACCTTCTGGGGTTGGT-3′ |
| G3bp1 | NM_013716.2 | 5′-GGGAAGCCAGCAGATGCAGT-3′ | 5′-ATCCACGTGGCGGATCTTG-3′ |
| Eif1a | NM_010120.3 | 5′-CCGCTGCGTTTTGGTCACTA-3′ | 5′-TCGGTTCTGGCCTGGTTCTC-3′ |
| Tubb5 | NM_011655.3 | 5′-CAGCTGGACCGAATCTCTGTGT-3′ | 5′-GGACCTGAGCGAACGGAGTC-3′ |
| Hmga2 | NM_010441.1 | 5′-GGTGCCACAGAAGCGAGGAC-3′ | 5′-GGGCTCACAGGTTGGCTCTT-3′ |
| Psap | NM_011179.2 | 5′-ACTGTGGGGCCGTGAAGC-3′ | 5′-GTCGCAAGGAAGGGATTTCG-3′ |
| Prnp | NM_011170.1 | 5′-TCCAATTTAGGAGAGCCAAGCA-3′ | 5′-GCCGACATCAGTCCACATAGTCA-3′ |
| Pld3 | NM_0111161.1 | 5′-GCTGAGGAACCGGAAGCTGT-3′ | 5′-GGGAAAGGGGTGGTCCTGAG-3′ |
| Rhob | NM_007483.2 | 5′-GGGGCACGCAGAGTGGTT-3′ | 5′-GCAACAGTAGTGGCTTGCTGGTT-3′ |
| Vwf | NM_011708.3 | 5′-GGCGAGGATGGAGTTCGACA-3′ | 5′-TGACAGGGCTGATGGTCTGG-3′ |
| Car3 | NM_007606.3 | 5′-TGGCTAAGGAGTGGGGCTAC-3′ | 5′-GTCCCCTTTGGCAATTGGAT-3′ |
| Plp1 | NM_011123.2 | 5′-TCAGTCTATTGCCTTCCCTAGCAA-3′ | 5′-GCATTCCATGGGAGAACACCA-3′ |
| Hdac3 | NM_010411 | 5′-TCAACGTGGGTGATGACTGC-3′ | 5′-GCAGAGATGCGCCTGTGTA-3′ |
| Chd4 | NM_145979 | 5′-GCTGCCAGAGATCCCAAACG-3′ | 5′-TTGCCCTTAAGAGCTGGACAA-3′ |
| Cul4a | NM_146207 | 5′-GGAGAACATTTGACAGCAATTCTACA-3′ | 5′-GTGAGGTCGGGCACCCTGT-3′ |
| Trim27 | NM_009054 | 5′-GAAGAGACGGCGGGCACA-3′ | 5′-GCTGCTCAAACTCCCAGACAA-3′ |
| Fus | NM_139149 | 5′-CACTGGTTGCATTCATTTCTCCA-3′ | 5′-CCAGTGGAGGTGGTGGAGGT-3′ |
| Cebpa | NM_007678 | 5′-CCAGTCAGACCAGAAAGCTGA-3′ | 5′-CCACAAAGCCCAGAAACCTA-3′ |
| Mlx | NM_011550 | 5′-TGTGTCTTCAGCTGGATTGAGGA-3′ | 5′-GGACACCGATCACAATCTCTCG-3′ |
| Smarcc1 | NM_009211 | 5′-TGGGAGAGCCCGGACACG-3′ | 5′-TTGGTAGGAGCATCTGCATGAAC-3′ |
| Smarcc2 | NM_198160 | 5′-TCTTCAGCCGAAGCCTCCAC-3′ | 5′-CCCTTCTCAGGGAAGTTCAGCA-3′ |
| Cdkn1a | NM_007669 | 5′-GTACTTCCTCTGCCCTGCTG-3′ | 5′-TCTGCGCTTGGAGTGATAGA-3′ |
| 1200009F10Rik | NM_026166 | 5′-GAAAAACAATACCGGTTACTGCAAA-3′ | 5′-CCACGAGAGCTTCACATTCCTG-3′ |
| Ezh2 | NM_007971.1 | 5′-CGCTCTTCTGTCGACGATGTTTT-3′ | 5′-GTTGGGTGTTGCATGGAAGG-3′ |
| Gata3 | NM_008091.2 | 5′-GGTATCCTCCGACCCACCAC-3′ | 5′-CCAGCCAGGGCAGAGATCC-3′ |
| Bmi1 | NM_007552.3 | 5′-AAACCAGACCACTCCTGAACA-3′ | 5′-TCTTCTTCTCTTCATCTCATTTTTGA-3′ |
| Sh2b3 | NM_008507 | 5′-CAACACACACAAGGCTGTCA-3′ | 5′-CCTGTGCACAAGAACTACATCTG-3′ |
| Rae28 | NM_001042623 | 5′-CGCACATCATTGAAGGCTTTGTT-3′ | 5′-TTCTTTCAGGAACTGAGAACATCC-3′ |