Bone marrow blast count and peripheral blood frequency of WT1 tetramer+ CD3CD8+ T cells and cytokine response to ex vivo stimulation with WT1 peptide
| Blast count in BM at baseline . | Patient no. . | Baseline . | Week 18 . | Week 18 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tetramer . | IFNγ . | TNFα . | Tetramer . | IFNγ . | TNFα . | Tetramer . | IFNγ . | TNFα . | ||
| Low | ||||||||||
| 10 | 1 | 0.08 | 0 | 0 | 0.33 | 0 | 0 | 0.54 | 0.6 | 0.6 |
| 5 | 4 | 0.15 | 0 | 0 | 0.3 | 0 | 0 | 0.37 | 0.1 | 0.35 |
| 12.5 | 5 | 1.37* | 0 | 0 | 1.88* | 0 | 0 | 1.59* | 0.21 | 0.41 |
| 10 | 11 | 0.04 | 0 | 0 | 0.14 | 0 | 0 | 0.06 | na | na |
| 12 | 12 | 0.38* | 0.25 | 0 | 0.54* | 0 | 0.11 | 1.09 | 0 | 0.05 |
| 10 | 14 | 0.06 | 0 | 0 | 0.05 | 0 | 0 | 0.13 | 0 | 0 |
| 5 | 17 | 0.17 | 0 | 0 | 0.41 | 0 | 0 | 0.7 | 0.53 | 0 |
| 30 | 19 | 0.11 | 0 | 0 | 0.23 | 0.05 | 0 | 0.22 | 0 | 0 |
| High | ||||||||||
| 85 | 2 | 0.2 | 0 | 0 | 0.42 | 0 | 0.09 | na | na | na |
| 60 | 3 | 0.39* | 0.06 | 0 | 0.08 | 0 | 0 | na | na | na |
| 60 | 6 | 0 | 0 | 0 | 0.14 | 0 | 0 | na | na | na |
| 70 | 7 | 0.11 | 0 | 0 | 0.14 | 0 | 0 | 0.23 | 0 | 0 |
| na | 8 | 0.11 | 0 | 0 | 0.28 | 0.22 | 0.1 | 0.22 | 0 | 0 |
| 80 | 9 | 2.0* | 0 | 0 | 0.92* | 0.13 | 0.11 | 1.11* | 0 | 0 |
| 60 | 13 | 0.12 | 0 | 0 | 0.33 | 0 | 0 | 0.26 | 0 | 0 |
| 60 | 15 | 0.1 | 0 | 0 | 0.09 | 0 | 0.12 | 0.14 | 0.09 | 0 |
| 50 | 16 | 0.13 | 0 | 0 | 0.27 | 0 | 0.25 | 0.14 | 0 | 0 |
| 40 | 18 | 0.1 | 0 | 0 | 0.12 | 0 | 0 | na | na | na |
| Blast count in BM at baseline . | Patient no. . | Baseline . | Week 18 . | Week 18 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tetramer . | IFNγ . | TNFα . | Tetramer . | IFNγ . | TNFα . | Tetramer . | IFNγ . | TNFα . | ||
| Low | ||||||||||
| 10 | 1 | 0.08 | 0 | 0 | 0.33 | 0 | 0 | 0.54 | 0.6 | 0.6 |
| 5 | 4 | 0.15 | 0 | 0 | 0.3 | 0 | 0 | 0.37 | 0.1 | 0.35 |
| 12.5 | 5 | 1.37* | 0 | 0 | 1.88* | 0 | 0 | 1.59* | 0.21 | 0.41 |
| 10 | 11 | 0.04 | 0 | 0 | 0.14 | 0 | 0 | 0.06 | na | na |
| 12 | 12 | 0.38* | 0.25 | 0 | 0.54* | 0 | 0.11 | 1.09 | 0 | 0.05 |
| 10 | 14 | 0.06 | 0 | 0 | 0.05 | 0 | 0 | 0.13 | 0 | 0 |
| 5 | 17 | 0.17 | 0 | 0 | 0.41 | 0 | 0 | 0.7 | 0.53 | 0 |
| 30 | 19 | 0.11 | 0 | 0 | 0.23 | 0.05 | 0 | 0.22 | 0 | 0 |
| High | ||||||||||
| 85 | 2 | 0.2 | 0 | 0 | 0.42 | 0 | 0.09 | na | na | na |
| 60 | 3 | 0.39* | 0.06 | 0 | 0.08 | 0 | 0 | na | na | na |
| 60 | 6 | 0 | 0 | 0 | 0.14 | 0 | 0 | na | na | na |
| 70 | 7 | 0.11 | 0 | 0 | 0.14 | 0 | 0 | 0.23 | 0 | 0 |
| na | 8 | 0.11 | 0 | 0 | 0.28 | 0.22 | 0.1 | 0.22 | 0 | 0 |
| 80 | 9 | 2.0* | 0 | 0 | 0.92* | 0.13 | 0.11 | 1.11* | 0 | 0 |
| 60 | 13 | 0.12 | 0 | 0 | 0.33 | 0 | 0 | 0.26 | 0 | 0 |
| 60 | 15 | 0.1 | 0 | 0 | 0.09 | 0 | 0.12 | 0.14 | 0.09 | 0 |
| 50 | 16 | 0.13 | 0 | 0 | 0.27 | 0 | 0.25 | 0.14 | 0 | 0 |
| 40 | 18 | 0.1 | 0 | 0 | 0.12 | 0 | 0 | na | na | na |
IFNγ and TNFα indicate intracellular cytokine response as detected by flow cytometry after stimulation with WT1 peptide in percent (%) of CD3+CD8+ T cells (a cytokine response was considered positive—printed in bold—if the percentage of WT1 peptide–specific cytokine-producing CD3+CD8+ T cells was at least 2-fold the percentage of an HIV control peptide; na, not available; Patient no., sequential number of inclusion into study; and tetramer indicates WT1-specific tetramer-positive T cells in percent (%) of CD3+CD8+ T cells, which were considered positive (indicated by an asterisk [*]), if the frequency of tetramer-positive CD3+CD8+ T cells at baseline exceeded 0.3%, which was the mean plus 2 standard deviations (0.16% + 0.14%) observed in 12 healthy control subjects: an increase of at least 2-fold in frequency as compared to baseline was considered as vaccine-induced response (indicated in bold).