T cell subset characteristics
| . | CD4+ T-helper cells . | CD8+ cytotoxic T cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| Naive . | Central memory . | Effector memory . | Effector . | Naive . | Central memory . | Effector memory . | Effector . | |
| CD62L | + | + | − | − | + | + | − | − |
| CD45RA | + | − | − | + | + | − | − | + |
| Changes from night to day†, % | −41 | −35 | −32 | NS | −46 | −30 | −23 | 23 |
| Correlation with cortisol‡ | −0.65* | −0.62* | −0.54* | NS | −0.68* | −0.54* | −0.42* | NS |
| Changes after cortisol§, % | −42 | −38 | −35 | NS | −39 | −27 | −21 | NS |
| GR expression‖ | 1063 ± 31* | 1098 ± 34*** | 1167 ± 35 | 1181 ± 36 | 1168 ± 33 | 1228 ± 50* | 1280 ± 51** | 1367 ± 55 |
| Correlation with epinephrine‡ | NS | NS | NS | NS | NS | NS | NS | 0.26* |
| Changes after epinephrine§, % | NS | NS | NS | NS | NS | NS | NS | 30 |
| Naive and central memory | Effector memory and effector | Naive and central memory | Effector memory | Effector | ||||
| β-AR expression¶ | 197 ± 42* | 827 ± 239 | 727 ± 181** | 2045 ± 579 | 2112 ± 602 | |||
| . | CD4+ T-helper cells . | CD8+ cytotoxic T cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| Naive . | Central memory . | Effector memory . | Effector . | Naive . | Central memory . | Effector memory . | Effector . | |
| CD62L | + | + | − | − | + | + | − | − |
| CD45RA | + | − | − | + | + | − | − | + |
| Changes from night to day†, % | −41 | −35 | −32 | NS | −46 | −30 | −23 | 23 |
| Correlation with cortisol‡ | −0.65* | −0.62* | −0.54* | NS | −0.68* | −0.54* | −0.42* | NS |
| Changes after cortisol§, % | −42 | −38 | −35 | NS | −39 | −27 | −21 | NS |
| GR expression‖ | 1063 ± 31* | 1098 ± 34*** | 1167 ± 35 | 1181 ± 36 | 1168 ± 33 | 1228 ± 50* | 1280 ± 51** | 1367 ± 55 |
| Correlation with epinephrine‡ | NS | NS | NS | NS | NS | NS | NS | 0.26* |
| Changes after epinephrine§, % | NS | NS | NS | NS | NS | NS | NS | 30 |
| Naive and central memory | Effector memory and effector | Naive and central memory | Effector memory | Effector | ||||
| β-AR expression¶ | 197 ± 42* | 827 ± 239 | 727 ± 181** | 2045 ± 579 | 2112 ± 602 | |||
Columns show data of naive (CD45RA+CD62L+), central memory (CD45RA−CD62L+), effector memory (CD45RA−CD62L−), and effector (CD45RA+CD62L−) CD4+ and CD8+ T cells.
NS indicates not significant.
Percentage changes comparing the two extremes of the circadian rhythm during nighttime and daytime.
Correlations assessed by stepwise linear regression analyses between circadian variation in cortisol/epinephrine and respective T cell subpopulation, measured with a delay (time lag) of 3 and 0 hours with reference to cortisol and epinephrine, respectively. *P < .001.
Percentage change in cell numbers 180 and 30 minutes after starting cortisol and epinephrine infusions, respectively, compared with placebo numbers (Figure 4I,J); n = 10.
Morning levels of GR expression in MFI, mean ± SEM; n = 8. *P < .05, **P < .01, ***P < .001, pairwise comparisons between naive versus central memory, central memory versus effector memory, and effector memory versus effector T cells, respectively.
Morning levels of β-AR expression measured by specific binding of 125I-CYP in isolated T cells; data of saturation curves (Figure 5) were analyzed using nonlinear regression plot, and the maximum number of 125I-CYP binding sites (Bmax) is presented as mean ± SEM; n = 6. *P < .05, **P < .01, pairwise comparisons between CD62L+ versus CD62L− T cells and effector memory versus effector CD8+ T cells, respectively.