Summary of cathepsin and cystatin gene and protein differential expression and enzymatic activity in HIV-1–treated MDDCs
| Name . | Microarray (FC) . | QPCR (FC) . | Western blot . | Function . | ||
|---|---|---|---|---|---|---|
| Viable . | Inactivated . | Viable . | Inactivated . | |||
| Cathepsin B | – | – | −3.5 | No change | ↓↓↓ | ↓↓↓ |
| Cathepsin C | −1.8 | No change | −2.0 | No change | ↓↓↓ | ↓↓ |
| Cathepsin Z | – | – | −2.6 | No change | ↓↓↓ | ↓↓↓ |
| Cathepsin S | −1.3 | No change | −1.8 | No change | ↓↓ | ↓↓ |
| Cathepsin L | +2.1 | +1.6 | +5.0 | +1.5 | No change | ↓↓↓ (BZLS) |
| Cathepsin H | +1.8 | +1.5 | +1.9 | +1.9 | No change | – |
| Cystatin C | −1.9 | No change | −2.5 | −2.1 | ↓↓ | – |
| Cystatin F | +2.3 | +1.5 | +3.0 | +1.5 | ↑ | – |
| Cystatin A | +1.9 | No change | +2.1 | No change | No change | – |
| Name . | Microarray (FC) . | QPCR (FC) . | Western blot . | Function . | ||
|---|---|---|---|---|---|---|
| Viable . | Inactivated . | Viable . | Inactivated . | |||
| Cathepsin B | – | – | −3.5 | No change | ↓↓↓ | ↓↓↓ |
| Cathepsin C | −1.8 | No change | −2.0 | No change | ↓↓↓ | ↓↓ |
| Cathepsin Z | – | – | −2.6 | No change | ↓↓↓ | ↓↓↓ |
| Cathepsin S | −1.3 | No change | −1.8 | No change | ↓↓ | ↓↓ |
| Cathepsin L | +2.1 | +1.6 | +5.0 | +1.5 | No change | ↓↓↓ (BZLS) |
| Cathepsin H | +1.8 | +1.5 | +1.9 | +1.9 | No change | – |
| Cystatin C | −1.9 | No change | −2.5 | −2.1 | ↓↓ | – |
| Cystatin F | +2.3 | +1.5 | +3.0 | +1.5 | ↑ | – |
| Cystatin A | +1.9 | No change | +2.1 | No change | No change | – |
The table summarizes the differential expression of genes encoding cathepsin and cystatin as well as associated protein levels and the enzymatic activity of cathepsins in MDDCs treated with HIV-1BAL (MOI1) for 48 hours. If a gene was not spotted on the microarray or a function assay was not performed, then this is marked with a dash.