Levels of erythroid (α- and β-globin and carbonic anydrase 1, Car1)– and MK (acethyl cholinesterase, Ache, platelet factor 4, PF4, and P-selectin)–specific genes expressed by immature (CD71high) and mature (CD71med) erythroblasts and by MKs isolated from the bone marrow and the spleen of 2.7Kb−Gata1GFPGata1+/0 and −2.7KbGata1GFPGata1low/0 males, as indicated
| . | 12.7Kb−Gata1GFPGata1+/0 . | 12.7Kb−Gata1GFPGata1low/0 . | ||
|---|---|---|---|---|
| Bone marrow mRNA, 2−ΔCt . | Spleen mRNA, 2−ΔCt . | Bone marrow mRNA, 2−ΔCt . | Spleen mRNA, 2−ΔCt . | |
| Erythroid cells | ||||
| CD71high | ||||
| Gata1, × 10−1 | 2.7 ± 0.3 | 2.1 ± 0.5 | 2.0 ± 0.1 | 0.5 ± 0.2 (down arrow) |
| Gata2, × 10−3 | 18.0 ± 2.0 | 6.0 ± 4.0 (down arrow) | 31.0 ± 2.0 | 0.8 ± 0.6 (down arrow) |
| α-globin, × 102 | 3.7 ± 0.3 | 6.7 ± 1.8 | 11.8 ± 1.2 | 11.5 ± 1.3 |
| β-globin, × 102 | 3.5 ± 0.2 | 4.6 ± 0.4 | 27.8 ± 2.4 | 9.3 ± 0.2 (down arrow) |
| Car 1, × 10−1 | 3.2 ± 0.7 | 1.2 ± 0.5 (down arrow) | 1.2 ± 0.5 | 0.4 ± 0.1 (down arrow) |
| CD71med | ||||
| Gata1, × 10−1 | 16.4 ± 2.0 | 4.1 ± 0.3 (down arrow) | 3.7 ± 0.3 | 4.7 ± 3.1(up arrow) |
| Gata2, × 10−3 | 1.0 ± 0.8 | 0.4 ± 0.1 | 0.9 ± 0.1 | 0.2 ± 0.1 |
| α-globin, × 102 | 3.1 ± 1.4 | 4.1 ± 2.1 | 8.7 ± 0.8 | 18.1 ± 1.8 (up arrow) |
| β-globin, × 102 | 3.0 ± 1.1 | 4.0 ± 1.1 | 8.5 ± 0.6 | 12.3 ± 1.2 (up arrow) |
| Car1, × 10−1 | 39.7 ± 6.4 | 20.1 ± 5.8 (down arrow) | 41.0 ± 4.0 | 19.0 ± 2.0 (down arrow) |
| Megakaryocytes (CD61posCD41pos) | ||||
| Gata1, × 10−1 | 0.5 ± 0.3 | 0.28 ± 0.05 | 0.01 ± 0.01 | 0.12 ± 0.03 (up arrow) |
| Gata2, × 10−3 | 38 ± 6 | 45 ± 2 | 47 ± 20 | 40 ± 8 |
| Ache, × 10−3 | 0.6 ± 0.3 | 2.0 ± 0.2 | 2.9 ± 0.4 | 5.4 ± 0.6 (up arrow) |
| PF4, × 10−2 | 5.1 ± 1.0 | 3.4 ± 0.8 | 3.2 ± 1.0 | 27.0 ± .44 (up arrow) |
| VWF, × 10−2 | 1.5 ± 0.3 | 1.3 ± 0.2 | 1.3 ± 0.3 | 4.9 ± 0.8 (up arrow) |
| p-Selectin, × 10−1 | 0.9 ± 0.5 | 4.7 ± 0.5 (up arrow) | 5.1 ± 0.9 | 2.7 ± 0.7 |
| α-globin, × 102 | 0.03 ± 0.01 | 0.07 ± 0.03 (up arrow) | 0.09 ± 0.03 | 0.5 ± 0.1 (up arrow) |
| β-globin, × 102 | 0.06 ± 0.001 | 0.07 ± 0.02 (up arrow) | 0.09 ± 0.2 | 0.5 ± 0.1 (up arrow) |
| Car1, × 10−1 | 0.09 ± 0.01 | 0.26 ± 0.13 (up arrow) | 0.39 ± 0.12 | 0.68 ± 0.38 |
| . | 12.7Kb−Gata1GFPGata1+/0 . | 12.7Kb−Gata1GFPGata1low/0 . | ||
|---|---|---|---|---|
| Bone marrow mRNA, 2−ΔCt . | Spleen mRNA, 2−ΔCt . | Bone marrow mRNA, 2−ΔCt . | Spleen mRNA, 2−ΔCt . | |
| Erythroid cells | ||||
| CD71high | ||||
| Gata1, × 10−1 | 2.7 ± 0.3 | 2.1 ± 0.5 | 2.0 ± 0.1 | 0.5 ± 0.2 (down arrow) |
| Gata2, × 10−3 | 18.0 ± 2.0 | 6.0 ± 4.0 (down arrow) | 31.0 ± 2.0 | 0.8 ± 0.6 (down arrow) |
| α-globin, × 102 | 3.7 ± 0.3 | 6.7 ± 1.8 | 11.8 ± 1.2 | 11.5 ± 1.3 |
| β-globin, × 102 | 3.5 ± 0.2 | 4.6 ± 0.4 | 27.8 ± 2.4 | 9.3 ± 0.2 (down arrow) |
| Car 1, × 10−1 | 3.2 ± 0.7 | 1.2 ± 0.5 (down arrow) | 1.2 ± 0.5 | 0.4 ± 0.1 (down arrow) |
| CD71med | ||||
| Gata1, × 10−1 | 16.4 ± 2.0 | 4.1 ± 0.3 (down arrow) | 3.7 ± 0.3 | 4.7 ± 3.1(up arrow) |
| Gata2, × 10−3 | 1.0 ± 0.8 | 0.4 ± 0.1 | 0.9 ± 0.1 | 0.2 ± 0.1 |
| α-globin, × 102 | 3.1 ± 1.4 | 4.1 ± 2.1 | 8.7 ± 0.8 | 18.1 ± 1.8 (up arrow) |
| β-globin, × 102 | 3.0 ± 1.1 | 4.0 ± 1.1 | 8.5 ± 0.6 | 12.3 ± 1.2 (up arrow) |
| Car1, × 10−1 | 39.7 ± 6.4 | 20.1 ± 5.8 (down arrow) | 41.0 ± 4.0 | 19.0 ± 2.0 (down arrow) |
| Megakaryocytes (CD61posCD41pos) | ||||
| Gata1, × 10−1 | 0.5 ± 0.3 | 0.28 ± 0.05 | 0.01 ± 0.01 | 0.12 ± 0.03 (up arrow) |
| Gata2, × 10−3 | 38 ± 6 | 45 ± 2 | 47 ± 20 | 40 ± 8 |
| Ache, × 10−3 | 0.6 ± 0.3 | 2.0 ± 0.2 | 2.9 ± 0.4 | 5.4 ± 0.6 (up arrow) |
| PF4, × 10−2 | 5.1 ± 1.0 | 3.4 ± 0.8 | 3.2 ± 1.0 | 27.0 ± .44 (up arrow) |
| VWF, × 10−2 | 1.5 ± 0.3 | 1.3 ± 0.2 | 1.3 ± 0.3 | 4.9 ± 0.8 (up arrow) |
| p-Selectin, × 10−1 | 0.9 ± 0.5 | 4.7 ± 0.5 (up arrow) | 5.1 ± 0.9 | 2.7 ± 0.7 |
| α-globin, × 102 | 0.03 ± 0.01 | 0.07 ± 0.03 (up arrow) | 0.09 ± 0.03 | 0.5 ± 0.1 (up arrow) |
| β-globin, × 102 | 0.06 ± 0.001 | 0.07 ± 0.02 (up arrow) | 0.09 ± 0.2 | 0.5 ± 0.1 (up arrow) |
| Car1, × 10−1 | 0.09 ± 0.01 | 0.26 ± 0.13 (up arrow) | 0.39 ± 0.12 | 0.68 ± 0.38 |
Results are expressed in 2−ΔCt and are presented as mean (± SD) level of expression in different populations isolated by FACS (> 90% pure by reanalysis) in at least 3 separate experiments. The expression of MK-specific genes in erythroid cells is not reported because it was below 10−4. Values statistically different (P<.01) from those expressed by the corresponding Gata1+/0 cells are indicated in red, those statistically different from the corresponding population in the marrow are indicated by down arrow (decreased) and up arrow (increased), whereas differences in expression between immature and mature erythroblasts are indicated in bold.