Primer sequences used for RT-PCR
| Gene/primer sequence . | Product, bp . | Accession no. . |
|---|---|---|
| GAPDH | ||
| 5′-GCAGTGGCAAAGTGGAGATT-3′ | 308 | XM_973383 |
| 5′-GCAGAAGGGGCGGAGATGAT-3′ | ||
| Collagen I | ||
| 5′-GCAATCGGGATCAGTACGAA-3′ | 485 | BC007158 |
| 5′-CTTTCACGCCTTTGAAGCCA-3′ | ||
| Osteocalcin | ||
| 5′-CTCTGTCTCTCTGACCTCACAG-3′ | 359 | L24430 |
| 5′-GGAGCTGCTGTGACATCCATAC-3′ | ||
| Ki-67 | ||
| 5′-CAGACGAGCAAGAGACAAAG-3′ | 282 | X82786 |
| 5′-AAGTACGGAGCCTGTATCAC-3′ | ||
| SDF-1 | ||
| 5′-AGTAAGCACAACAGCCCAAAGG-3′ | 71 | L12029 |
| 5′-CTTGCATCTCCCACGGATGT-3′ | ||
| CXCR4 | ||
| 5′-ACCATCTACTTCATCATCTTC-3′ | 480 | NM_009911 |
| 5′-CACCATCCACAGGCTATC-3′ | ||
| PDGF-β | ||
| 5′-TGGATTAGAGGTGCAGTGTG-3′ | 366 | NM_01105 |
| 5′-TCCCTTTCCTCAGAGGTTTG-3′ | ||
| TGF-β1 | ||
| 5′-CACCTGCAAGACCATCGACA-3′ | 241 | BC013738 |
| 5′-CACGCGGGTGACCTCTTTAG-3′ | ||
| bFGF | ||
| 5′-CCCACACGTCAAACTACAACT-3′ | 298 | NM_008006.2 |
| 5′-TCAGCTCTTAGCAGACATTGG-3′ | ||
| Osteopontin | ||
| 5′-TCACCATTCGGATGAGTCTG-3′ | 437 | NM_009263 |
| 5′-ACTTGTGGCTCTGATGTTCC-3′ | ||
| N-cadherin | ||
| 5′-ACGAGAGGCCTATCCATGCTGAGC-3′ | 293 | AB008811 |
| 5′-TCAGTCGTCACCACCGCCGTACAT-3′ | ||
| VE-cadherin | ||
| 5′-GCAGAGGCTCACAGAGCTGG-3′ | 700 | NM_009868 |
| 5′-CGCAGACCCAGGGCATCTTG-3′ | ||
| Factor VIII | ||
| 5′-ATGCAAATAGCACTCTTCGCTTG-3′ | 799 | NM_007977 |
| 5′-AATCAGACCTGGAAGAGACCTG-3′ |
| Gene/primer sequence . | Product, bp . | Accession no. . |
|---|---|---|
| GAPDH | ||
| 5′-GCAGTGGCAAAGTGGAGATT-3′ | 308 | XM_973383 |
| 5′-GCAGAAGGGGCGGAGATGAT-3′ | ||
| Collagen I | ||
| 5′-GCAATCGGGATCAGTACGAA-3′ | 485 | BC007158 |
| 5′-CTTTCACGCCTTTGAAGCCA-3′ | ||
| Osteocalcin | ||
| 5′-CTCTGTCTCTCTGACCTCACAG-3′ | 359 | L24430 |
| 5′-GGAGCTGCTGTGACATCCATAC-3′ | ||
| Ki-67 | ||
| 5′-CAGACGAGCAAGAGACAAAG-3′ | 282 | X82786 |
| 5′-AAGTACGGAGCCTGTATCAC-3′ | ||
| SDF-1 | ||
| 5′-AGTAAGCACAACAGCCCAAAGG-3′ | 71 | L12029 |
| 5′-CTTGCATCTCCCACGGATGT-3′ | ||
| CXCR4 | ||
| 5′-ACCATCTACTTCATCATCTTC-3′ | 480 | NM_009911 |
| 5′-CACCATCCACAGGCTATC-3′ | ||
| PDGF-β | ||
| 5′-TGGATTAGAGGTGCAGTGTG-3′ | 366 | NM_01105 |
| 5′-TCCCTTTCCTCAGAGGTTTG-3′ | ||
| TGF-β1 | ||
| 5′-CACCTGCAAGACCATCGACA-3′ | 241 | BC013738 |
| 5′-CACGCGGGTGACCTCTTTAG-3′ | ||
| bFGF | ||
| 5′-CCCACACGTCAAACTACAACT-3′ | 298 | NM_008006.2 |
| 5′-TCAGCTCTTAGCAGACATTGG-3′ | ||
| Osteopontin | ||
| 5′-TCACCATTCGGATGAGTCTG-3′ | 437 | NM_009263 |
| 5′-ACTTGTGGCTCTGATGTTCC-3′ | ||
| N-cadherin | ||
| 5′-ACGAGAGGCCTATCCATGCTGAGC-3′ | 293 | AB008811 |
| 5′-TCAGTCGTCACCACCGCCGTACAT-3′ | ||
| VE-cadherin | ||
| 5′-GCAGAGGCTCACAGAGCTGG-3′ | 700 | NM_009868 |
| 5′-CGCAGACCCAGGGCATCTTG-3′ | ||
| Factor VIII | ||
| 5′-ATGCAAATAGCACTCTTCGCTTG-3′ | 799 | NM_007977 |
| 5′-AATCAGACCTGGAAGAGACCTG-3′ |