ProCISE assay parameters
| . | β5 . | β1 . | β2 . | LMP7 . | LMP2 . | MECL1 . |
|---|---|---|---|---|---|---|
| ULOQ,* μg/mL (20S) | 25 | 25 | 25 | 25 | 25 | 25 |
| LLOQ, μg/mL (20S) | 0.23 | 0.49 | 1.88 | 0.23 | 0.42 | 0.24 |
| Dynamic window† (20S) | ∼ 100 times | ∼ 50 times | ∼ 13 times | ∼ 100 times | ∼ 60 times | ∼ 100 times |
| % CV,‡ MM1.S | 9.8 | 7.8 | 11.2 | 14.0 | 13.3 | 11.9 |
| Quantitative range,§ MM1.S | 100% to 6.5% | 100% to 6.5% | 100% to 25% | 100% to 6.5% | 100% to 6.5% | 100% to 6.5% |
| . | β5 . | β1 . | β2 . | LMP7 . | LMP2 . | MECL1 . |
|---|---|---|---|---|---|---|
| ULOQ,* μg/mL (20S) | 25 | 25 | 25 | 25 | 25 | 25 |
| LLOQ, μg/mL (20S) | 0.23 | 0.49 | 1.88 | 0.23 | 0.42 | 0.24 |
| Dynamic window† (20S) | ∼ 100 times | ∼ 50 times | ∼ 13 times | ∼ 100 times | ∼ 60 times | ∼ 100 times |
| % CV,‡ MM1.S | 9.8 | 7.8 | 11.2 | 14.0 | 13.3 | 11.9 |
| Quantitative range,§ MM1.S | 100% to 6.5% | 100% to 6.5% | 100% to 25% | 100% to 6.5% | 100% to 6.5% | 100% to 6.5% |
ULOQ indicates upper limit of quantitation; and LLOQ, lower limit of quantitation.
Defined as points of the standard curve for which the 2-sided 95% confidence intervals are within ± 25% of nominal %RE.
Defined as the ratio of ULOQ to LLOQ.
Interday coefficient of variation for 1 mg/mL cell lysate from 4 independent experiments.
Refers to range of subunit activity that can be quantitatively measured.