Virus reactivation from myeloid progenitor cells infected with AD169 and RVAdIL10C by coculture with permissive HFs
| Day after coculture at which reactivation determined by the appearance of cytopathic effect (CPE) in HFs was first detected . | ||||||
|---|---|---|---|---|---|---|
| Day after infection . | AD169 infected myeloid progenitor cells . | RVAdIL10C infected myeloid progenitor cells . | AD169 infected myeloid progenitor cell lysate . | RVAdIL10C infected myeloid progenitor cell lysate . | Supernatant from AD169 infected myeloid progenitor cell culture . | Supernatant from RVAdIL10C infected myeloid progenitor cell culture . |
| 3 | 13.7 (2.7 × 10−4) | 14.3 (2.9 × 10−4) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) |
| 5 | 12.3 (2.5 × 10−4) | 12.3 (2.5 × 10−4) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) |
| 8 | 9.3 (1.6 × 10−4) | 10.0 (2.0 × 10−4) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) |
| Day after coculture at which reactivation determined by the appearance of cytopathic effect (CPE) in HFs was first detected . | ||||||
|---|---|---|---|---|---|---|
| Day after infection . | AD169 infected myeloid progenitor cells . | RVAdIL10C infected myeloid progenitor cells . | AD169 infected myeloid progenitor cell lysate . | RVAdIL10C infected myeloid progenitor cell lysate . | Supernatant from AD169 infected myeloid progenitor cell culture . | Supernatant from RVAdIL10C infected myeloid progenitor cell culture . |
| 3 | 13.7 (2.7 × 10−4) | 14.3 (2.9 × 10−4) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) |
| 5 | 12.3 (2.5 × 10−4) | 12.3 (2.5 × 10−4) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) |
| 8 | 9.3 (1.6 × 10−4) | 10.0 (2.0 × 10−4) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) | No CPE (< 1.0 × 10−5) |
Values represent the average from 3 independent replicate experiments. Values in parentheses indicate the average frequency of myeloid progenitors that reactivated to produce infectious virus determined by infectious center assay.
CPE indicates cytopathic effect; and HFs, human foreskin fibroblasts.
No CPE indicates no cytopathic effect observed after 30 days of coculture with permissive HFs.