Host asmase mediates graft-versus-host target organ injury
| Transplantation . | mHA-disparate model (LP→B6) . | MHC-disparate model (B10.BR→B6) . | ||
|---|---|---|---|---|
| Liver . | Small intestine . | Liver . | Small intestine . | |
| BM (asmase+/+ recipients) | 6.3 ± 2.7 | 3.5 ± 0.5 | 1.0 ± 0.0 | 2.0 ± 0.0 |
| BM (asmase−/− recipients) | 5.1 ± 0.9 | 3.1 ± 0.4 | 0.7 ± 0.3 | 1.5 ± 0.5 |
| BM + T (asmase+/+ recipients) | 15.7 ± 1.5* | 10.7 ± 1.1* | 16.3 ± 1.1* | 10.0 ± 0.8* |
| BM + T (asmase−/− recipients) | 10.2 ± 0.5† | 7.0 ± 0.1† | 7.4 ± 0.9† | 3.5 ± 0.4† |
| Transplantation . | mHA-disparate model (LP→B6) . | MHC-disparate model (B10.BR→B6) . | ||
|---|---|---|---|---|
| Liver . | Small intestine . | Liver . | Small intestine . | |
| BM (asmase+/+ recipients) | 6.3 ± 2.7 | 3.5 ± 0.5 | 1.0 ± 0.0 | 2.0 ± 0.0 |
| BM (asmase−/− recipients) | 5.1 ± 0.9 | 3.1 ± 0.4 | 0.7 ± 0.3 | 1.5 ± 0.5 |
| BM + T (asmase+/+ recipients) | 15.7 ± 1.5* | 10.7 ± 1.1* | 16.3 ± 1.1* | 10.0 ± 0.8* |
| BM + T (asmase−/− recipients) | 10.2 ± 0.5† | 7.0 ± 0.1† | 7.4 ± 0.9† | 3.5 ± 0.4† |
C57BL/6 recipient hosts received LP BM and T cells (mHA disparity) as described in Figure 1, or alternatively, received 10 × 106 BM and 0.5 × 106 T cells from B10.BR donors (MHC disparity). asmase+/+ and asmase−/− recipients were killed 21 (mHA disparate model) or 14 (MHC disparate model) days after BMT, and tissues were harvested for histopathologic analysis. Small intestine and liver were scored for established organ-specific parameters in a blinded fashion (mean ± SEM) for 4 to 14 animals per group, as described in “Assessment of GVHD.”
P < .01 versus BM group (asmase+/+ hosts).
P < .01 versus BM+ T (asmase+/+ hosts).