Host asmase regulates graft-versus-host–induced serum cytokine elevation
| . | BM asmase+/+ recipients . | BM asmase−/− recipients . | BM + T asmase+/+ recipients . | BM + T asmase−/− recipients . |
|---|---|---|---|---|
| mHA disparate model (LP→B6) | ||||
| IL-2, pg/mL | 15.7 ± 4.1 | 13.0 ± 1.6 | 30.3 ± 2.8* | 19.1 ± 2.0† |
| IFN-γ, pg/mL | 3.7 ± 1.0 | 1.3 ± 0.4 | 109.9 ± 18.9* | 32.8 ± 12.4† |
| IL-1β, fg/mL | 6.8 ± 1.9 | 5.5 ± 0.5 | 13.4 ± 1.1* | 2.9 ± 1.2† |
| TNF-α, pg/mL | 2.3 ± 1.2 | 5.2 ± 1.0 | 24.3 ± 7.18 | 17.3 ± 3.0 |
| MHC disparate model (B10.BR→B6) | ||||
| IFN-γ, pg/mL | 4.1 ± 1.0 | 3.6 ± 1.9 | 199.1 ± 14.2* | 63.3 ± 10.8† |
| TNF-α, pg/mL | 33.0 ± 1.9 | 16.7 ± 2.3 | 136.8 ± 11.5* | 63.7 ± 9.0† |
| . | BM asmase+/+ recipients . | BM asmase−/− recipients . | BM + T asmase+/+ recipients . | BM + T asmase−/− recipients . |
|---|---|---|---|---|
| mHA disparate model (LP→B6) | ||||
| IL-2, pg/mL | 15.7 ± 4.1 | 13.0 ± 1.6 | 30.3 ± 2.8* | 19.1 ± 2.0† |
| IFN-γ, pg/mL | 3.7 ± 1.0 | 1.3 ± 0.4 | 109.9 ± 18.9* | 32.8 ± 12.4† |
| IL-1β, fg/mL | 6.8 ± 1.9 | 5.5 ± 0.5 | 13.4 ± 1.1* | 2.9 ± 1.2† |
| TNF-α, pg/mL | 2.3 ± 1.2 | 5.2 ± 1.0 | 24.3 ± 7.18 | 17.3 ± 3.0 |
| MHC disparate model (B10.BR→B6) | ||||
| IFN-γ, pg/mL | 4.1 ± 1.0 | 3.6 ± 1.9 | 199.1 ± 14.2* | 63.3 ± 10.8† |
| TNF-α, pg/mL | 33.0 ± 1.9 | 16.7 ± 2.3 | 136.8 ± 11.5* | 63.7 ± 9.0† |
Serum was obtained from peripheral blood on day 7 after marrow transplantation by retro-orbital puncture from C57BL/6 recipients of LP/J TCD-BM with or without splenic T cells, or alternatively, recipients of 10 × 106 BM with or without 0.5 × 106 T cells from B10.BR donors. Serum proinflammatory cytokines were quantified by ELISA, as described in “Enzyme-linked immunosorbent assay.” Cytokine levels (mean ± SEM) compiled from 3 to 8 determinations from 3 independent experiments for each group are depicted.
P < .005 versus BM (asmase+/+ hosts).
P < .01 versus BM + T (asmase+/+ hosts).