B-cell subpopulations in WT and BCL10 Tg mice
| . | WT . | BCL10 Tg . |
|---|---|---|
| Bone marrow (× 105/femur)* | ||
| B cells (B220+) | 27.7 ± 2.7 | 24.1 ± 1.8 |
| Fr A B cells (B220+CD43+CD24a−CD13−) | 2.1 ± 0.1 | 2.7 ± 0.3† |
| Fr B/C B cells (B220+CD43+CD24a+CD13+/−) | 1.9 ± 0.3 | 1.1 ± 0.2 |
| Fr D pre-B cells (B220+IgM−CD43−/lo) | 12.4 ± 1.0 | 7.0 ± 1.1‡ |
| Fr E immature B cells (B220loIgM+) | 4.4 ± 0.4 | 3.4 ± 0.4 |
| Fr F recirculating B cells (B220hiIgM+) | 6.7 ± 1.0 | 9.8 ± 1.0† |
| Spleen* | ||
| Weight (mg) | 100 ± 10.0 | 140 ± 10.0‡ |
| Spleen/body weight ratio | 0.36 ± 0.03 | 0.48 ± 0.04† |
| Total B cells (% of total) | 54.5 ± 1.9 | 47.7 ± 4.0 |
| Transitional (B220+IgM+CD23−CD21−) | 2.9 ± 0.2 | 1.7 ± 0.2‡ |
| Follicular (B220+IgM+CD23+CD21−/lo) | 29.6 ± 1. | 19.1 ± 1.9‡ |
| MZ (B220+IgM+CD23−CD21+) | 9.4 ± 0.6 | 14.7 ± 1.6‡ |
| Peritoneum (× 105)§ | ||
| B cells (CD19+) | 4.5 ± 1.2 | 9.8 ± 1.8† |
| B2 (CD19+CD23+CD5−) | 2.9 ± 0.1 | 5.3 ± 0.7† |
| B1a (CD19+CD23−CD5+) | 1.1 ± 0.2 | 0.2 ± 0.1‡ |
| B1b (CD19+CD23−CD5−CD11b+) | 1.2 ± 0.3 | 2.8 ± 0.9 |
| . | WT . | BCL10 Tg . |
|---|---|---|
| Bone marrow (× 105/femur)* | ||
| B cells (B220+) | 27.7 ± 2.7 | 24.1 ± 1.8 |
| Fr A B cells (B220+CD43+CD24a−CD13−) | 2.1 ± 0.1 | 2.7 ± 0.3† |
| Fr B/C B cells (B220+CD43+CD24a+CD13+/−) | 1.9 ± 0.3 | 1.1 ± 0.2 |
| Fr D pre-B cells (B220+IgM−CD43−/lo) | 12.4 ± 1.0 | 7.0 ± 1.1‡ |
| Fr E immature B cells (B220loIgM+) | 4.4 ± 0.4 | 3.4 ± 0.4 |
| Fr F recirculating B cells (B220hiIgM+) | 6.7 ± 1.0 | 9.8 ± 1.0† |
| Spleen* | ||
| Weight (mg) | 100 ± 10.0 | 140 ± 10.0‡ |
| Spleen/body weight ratio | 0.36 ± 0.03 | 0.48 ± 0.04† |
| Total B cells (% of total) | 54.5 ± 1.9 | 47.7 ± 4.0 |
| Transitional (B220+IgM+CD23−CD21−) | 2.9 ± 0.2 | 1.7 ± 0.2‡ |
| Follicular (B220+IgM+CD23+CD21−/lo) | 29.6 ± 1. | 19.1 ± 1.9‡ |
| MZ (B220+IgM+CD23−CD21+) | 9.4 ± 0.6 | 14.7 ± 1.6‡ |
| Peritoneum (× 105)§ | ||
| B cells (CD19+) | 4.5 ± 1.2 | 9.8 ± 1.8† |
| B2 (CD19+CD23+CD5−) | 2.9 ± 0.1 | 5.3 ± 0.7† |
| B1a (CD19+CD23−CD5+) | 1.1 ± 0.2 | 0.2 ± 0.1‡ |
| B1b (CD19+CD23−CD5−CD11b+) | 1.2 ± 0.3 | 2.8 ± 0.9 |
The cell numbers (mean ± SE) were calculated from 6 to 9 mice based on cell count and flow cytometric analysis using gating schemes illustrated in Figure 1.
WT indicates wild-type; Tg, transgenic; Fr, fraction; and MZ, marginal zone.
Bone marrow (n = 9) and BCL10 Tg (n = 7).
P < .05 compared with WT controls.
P <.01 compared with WT controls.
Bone marrow (n = 7) and BCL10 Tg (n = 6).