Molecular characteristics of recombinant CLL mAbs used in these studies
| CLL no. . | Subset* . | IGHV . | Mut status . | Mut % . | IGHD . | IGHJ . | HCDR3 amino acid sequence . | IGH GenBank no. . | IGKV/IGLV . | IGLJ . | Mut, % . | LCDR3 amino acid sequence . | IGL GenBank no. . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 014 | 9 | 1-69 | U-CLL | 0.0 | 3-03 | 6 | ATKNDFWSGYYEGYYYYYYMDV | AF021951 | L3-01 | LJ1 | 0.0 | QAWDSSTCYV | AY043106 |
| 068 | 6 | 1-69 | U-CLL | 0.0 | 3-16 | 3 | ARGGDYDYVWGSYRSNDAFDI | AY553640 | K3-20 | KJ4 | 0.0 | QQYGSSPT | AY574935 |
| 114 | 8 | 4-39 | U-CLL | 0.7 | 6-13 | 5 | ARRFGYSSSWYGLDWFDP | AY268372 | K1-39/1D-39 | KJ1 | 0.7 | QQSYSTPRT | AY043094 |
| 270 | 1 | 1-02 | U-CLL | 0.0 | 5-12 | 4 | ARVQWLGLRHFDY | AY055487 | K1-39/1D-39 | KJ2 | 0.0 | QQSYSTPPYT | AY574940 |
| 169 | NA | 3-33 | M-CLL | 8.8 | 3-10 | 4 | AREGGVTGQGGFDY | AY055480 | K4-01 | KJ4 | 0.0 | QQYYSTPLT | FJ039794 |
| 183 | 4 | 4-34 | M-CLL | 3.1 | 5-18 | 6 | ARGYGDTPTIRRYYYYGMDV | AF021948 | K2-30 | KJ2 | 1.7 | MQGTHWPPYT | AY043097 |
| 255 | NA | 4-59 | M-CLL | 4.2 | 3-22 | 4 | ARHRGYESSGYYSSYFDY | FJ039782 | L3-01 | LJ1 | 2.2 | QAWDSSTVV | FJ039804 |
| 412 | 2 | 3-21 | M-CLL | 2.0 | IND | 6 | ARDQNGMDV | AY553648 | L3-21 | LJ3 | 0.7 | QVWDSSSDHPWV | AY574946 |
| CLL no. . | Subset* . | IGHV . | Mut status . | Mut % . | IGHD . | IGHJ . | HCDR3 amino acid sequence . | IGH GenBank no. . | IGKV/IGLV . | IGLJ . | Mut, % . | LCDR3 amino acid sequence . | IGL GenBank no. . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 014 | 9 | 1-69 | U-CLL | 0.0 | 3-03 | 6 | ATKNDFWSGYYEGYYYYYYMDV | AF021951 | L3-01 | LJ1 | 0.0 | QAWDSSTCYV | AY043106 |
| 068 | 6 | 1-69 | U-CLL | 0.0 | 3-16 | 3 | ARGGDYDYVWGSYRSNDAFDI | AY553640 | K3-20 | KJ4 | 0.0 | QQYGSSPT | AY574935 |
| 114 | 8 | 4-39 | U-CLL | 0.7 | 6-13 | 5 | ARRFGYSSSWYGLDWFDP | AY268372 | K1-39/1D-39 | KJ1 | 0.7 | QQSYSTPRT | AY043094 |
| 270 | 1 | 1-02 | U-CLL | 0.0 | 5-12 | 4 | ARVQWLGLRHFDY | AY055487 | K1-39/1D-39 | KJ2 | 0.0 | QQSYSTPPYT | AY574940 |
| 169 | NA | 3-33 | M-CLL | 8.8 | 3-10 | 4 | AREGGVTGQGGFDY | AY055480 | K4-01 | KJ4 | 0.0 | QQYYSTPLT | FJ039794 |
| 183 | 4 | 4-34 | M-CLL | 3.1 | 5-18 | 6 | ARGYGDTPTIRRYYYYGMDV | AF021948 | K2-30 | KJ2 | 1.7 | MQGTHWPPYT | AY043097 |
| 255 | NA | 4-59 | M-CLL | 4.2 | 3-22 | 4 | ARHRGYESSGYYSSYFDY | FJ039782 | L3-01 | LJ1 | 2.2 | QAWDSSTVV | FJ039804 |
| 412 | 2 | 3-21 | M-CLL | 2.0 | IND | 6 | ARDQNGMDV | AY553648 | L3-21 | LJ3 | 0.7 | QVWDSSSDHPWV | AY574946 |
Mut indicates mutation; U-CLL, CLL cells with unmutated IGHV; M-CLL, CLL cells with mutated IGHV; NA, not attributable to a currently defined stereotypic subset; and IND, indeterminate.
Subset according to Stamatopoulos et al.16