Oligonucleotide primers and probes (q3C)
| Designation . | Sequence . |
|---|---|
| Human primers | |
| 53 H3 P | TTTCTGGAGGAGGACACAACTGATTTCCC |
| HS48 H3 P | CAGGCCAAACATAGCAACACCATCCC |
| HS46 H3 P | AGTCTAGTCCCCTCAGCTTTTAGCAT |
| HS40 H3 P | CCTGATTGCTAAAGCCTTTTCACAGCTGG |
| HS33 H3 P | CAAGATGTGCAAGCCCACTGTGAT |
| HS10 H3 P | TGGTCTTTTGCGTTTGGTGACAGTCAA |
| a2 H3 P | CGGGAGCGATCTGGGTCGAGG |
| 189 H3 P | TCTCTCGGATCTGAATGAACCCCAAGTG |
| 380 H3 P | TCAATGCAAAGAAAACCATAGCCAATT |
| 153 H3 R | CACTATATTGGAAGACCATGCAACTC |
| 53 H3 A F | AAAGAAGGATGTGTCCAGCTCATC |
| HS48 H3 A R | AAGTGGACTGTGAAAGAAATAAGGAAA |
| HS46 H3 A R | TCACAGGTCCTGTCTAGTTCTAAGGTT |
| HS40 H3 A R | CATAAAGACACCACATGCACTAGCT |
| HS33 H3 A R | CTGGTATTTCTGCCATTTGGAAA |
| HS10 H3 A F | AACAGATGCCAGCCAAACAGA |
| 189 H3 A R | ACCTTGGTCTCCATGACAATGAC |
| 380 H3 A R | AGTTCGTTTTCCAGCAGTTTGACTA |
| a2 H3 A R | GAGAGGAAGGCGCCATCTC |
| 53 H3 F | AATCAGTTGTGTCCTCCTCCAGAA |
| HS48 H3 R | AATAAGGAAACTGGGATGGTGTTG |
| HS46 H3 R | GATGCTTTCTCAGATGCTAAAAGCT |
| HS40 H3 F | AGCTGTGAAAAGGCTTTAGCAATC |
| 109 H3 F | CCCTTATCCCAAACACTTAAACTTTC |
| HS33 H3 R | CTGGTATTTCTGCCATTTGGAAA |
| HS10 H3 F | GACTGTCACCAAACGCAAAAGAC |
| a2 H3 R | GCCGCTCACCTTGAAGTTGA |
| 189 H3 R | ACTTCGGTGGCAAGTTACACTTG |
| 218 H3 R | ACAGTCTTCGATAGAGTTTGAAAGCTT |
| 380 H3 R | TCTTTGCATTGATTCTTGTTAAGCTT |
| 401 H3 F | GCGATGACGCGGCACTT |
| 441 H3 F | AAACAGCCAAGTGGATACATTCAA |
| xpb H3 F | CGGTGAGGTGAGTTTGTGGAAT |
| xpb H3 R | AGGATCTCTGTTTAATGGAAAAGCTT |
| xpb H3 P | AAGGATGAAGGCGTGATCCGACTCTG |
| hPr/Ex1 F | GGGCCGGCACTCTTCTG |
| hPr/Ex1 R | GGCCTTGACGTTGGTCTTGT |
| hPr/Ex1 P | CCCACAGACTCAGAGAGAACCCACCATG |
| Mouse primers | |
| 24 H3 F | ATGGGACAATGGAGGGCTAAG |
| 55 H3 R | TCTATTAGGGATGGCACTTTACGAA |
| 72 H3 R | CCCCTGTGGGAAAGACAGAAA |
| 78 H3 F | AGACAAGGAAATGGAAGAGACATCA |
| HS31 H3 F | AGCTGTGCCTGGCAGATGAC |
| HS29 H3 F | AGGAAGAAGAGGTTCAACAAGCAT |
| HS26 H3 R | GAATCTCCATCTCCAAGGG |
| Zeta H3 F | ACAACGTGGAGAGGACTGAGAGAT |
| 174 H3 F | AATGGTTGCCTTTTAAAGACATTTGT |
| a2 H3 R | GTGGCCATGAGCCAAAGAAT |
| a2 H3 P | CCTCCAGGTACCATAAATCTCAGACTTG |
| xpb H3 F | CTTCTGCTACAAAGCGCTGACTT |
| xpb H3 R | TGCCCTCCCTGAAAATAAGGA |
| xpb H3 P | TGCACCCTGCTTTAGTGGCCTTGTAGG |
| mPr/Ex1Fw | TGGAGGGCATATAAGTGCTACTTG |
| mPr/EX1Rev | TGCTTTTGTCTTCCCCAGAGA |
| mPr/Ex1P | TGCAGGTCCAAGACACTTCTGATTCTGACA |
| Designation . | Sequence . |
|---|---|
| Human primers | |
| 53 H3 P | TTTCTGGAGGAGGACACAACTGATTTCCC |
| HS48 H3 P | CAGGCCAAACATAGCAACACCATCCC |
| HS46 H3 P | AGTCTAGTCCCCTCAGCTTTTAGCAT |
| HS40 H3 P | CCTGATTGCTAAAGCCTTTTCACAGCTGG |
| HS33 H3 P | CAAGATGTGCAAGCCCACTGTGAT |
| HS10 H3 P | TGGTCTTTTGCGTTTGGTGACAGTCAA |
| a2 H3 P | CGGGAGCGATCTGGGTCGAGG |
| 189 H3 P | TCTCTCGGATCTGAATGAACCCCAAGTG |
| 380 H3 P | TCAATGCAAAGAAAACCATAGCCAATT |
| 153 H3 R | CACTATATTGGAAGACCATGCAACTC |
| 53 H3 A F | AAAGAAGGATGTGTCCAGCTCATC |
| HS48 H3 A R | AAGTGGACTGTGAAAGAAATAAGGAAA |
| HS46 H3 A R | TCACAGGTCCTGTCTAGTTCTAAGGTT |
| HS40 H3 A R | CATAAAGACACCACATGCACTAGCT |
| HS33 H3 A R | CTGGTATTTCTGCCATTTGGAAA |
| HS10 H3 A F | AACAGATGCCAGCCAAACAGA |
| 189 H3 A R | ACCTTGGTCTCCATGACAATGAC |
| 380 H3 A R | AGTTCGTTTTCCAGCAGTTTGACTA |
| a2 H3 A R | GAGAGGAAGGCGCCATCTC |
| 53 H3 F | AATCAGTTGTGTCCTCCTCCAGAA |
| HS48 H3 R | AATAAGGAAACTGGGATGGTGTTG |
| HS46 H3 R | GATGCTTTCTCAGATGCTAAAAGCT |
| HS40 H3 F | AGCTGTGAAAAGGCTTTAGCAATC |
| 109 H3 F | CCCTTATCCCAAACACTTAAACTTTC |
| HS33 H3 R | CTGGTATTTCTGCCATTTGGAAA |
| HS10 H3 F | GACTGTCACCAAACGCAAAAGAC |
| a2 H3 R | GCCGCTCACCTTGAAGTTGA |
| 189 H3 R | ACTTCGGTGGCAAGTTACACTTG |
| 218 H3 R | ACAGTCTTCGATAGAGTTTGAAAGCTT |
| 380 H3 R | TCTTTGCATTGATTCTTGTTAAGCTT |
| 401 H3 F | GCGATGACGCGGCACTT |
| 441 H3 F | AAACAGCCAAGTGGATACATTCAA |
| xpb H3 F | CGGTGAGGTGAGTTTGTGGAAT |
| xpb H3 R | AGGATCTCTGTTTAATGGAAAAGCTT |
| xpb H3 P | AAGGATGAAGGCGTGATCCGACTCTG |
| hPr/Ex1 F | GGGCCGGCACTCTTCTG |
| hPr/Ex1 R | GGCCTTGACGTTGGTCTTGT |
| hPr/Ex1 P | CCCACAGACTCAGAGAGAACCCACCATG |
| Mouse primers | |
| 24 H3 F | ATGGGACAATGGAGGGCTAAG |
| 55 H3 R | TCTATTAGGGATGGCACTTTACGAA |
| 72 H3 R | CCCCTGTGGGAAAGACAGAAA |
| 78 H3 F | AGACAAGGAAATGGAAGAGACATCA |
| HS31 H3 F | AGCTGTGCCTGGCAGATGAC |
| HS29 H3 F | AGGAAGAAGAGGTTCAACAAGCAT |
| HS26 H3 R | GAATCTCCATCTCCAAGGG |
| Zeta H3 F | ACAACGTGGAGAGGACTGAGAGAT |
| 174 H3 F | AATGGTTGCCTTTTAAAGACATTTGT |
| a2 H3 R | GTGGCCATGAGCCAAAGAAT |
| a2 H3 P | CCTCCAGGTACCATAAATCTCAGACTTG |
| xpb H3 F | CTTCTGCTACAAAGCGCTGACTT |
| xpb H3 R | TGCCCTCCCTGAAAATAAGGA |
| xpb H3 P | TGCACCCTGCTTTAGTGGCCTTGTAGG |
| mPr/Ex1Fw | TGGAGGGCATATAAGTGCTACTTG |
| mPr/EX1Rev | TGCTTTTGTCTTCCCCAGAGA |
| mPr/Ex1P | TGCAGGTCCAAGACACTTCTGATTCTGACA |
Oligonucleotide sequences used in real-time PCR.
P indicates probe; R, reverse; A, anchor; and F, forward.