IGF-IRα, IGF-IRβ, and IGF-I primers and PCR conditions
| Primers and conditions . | . |
|---|---|
| IGF-IRα (product size 307 bp) | |
| Forward | 5′-GTAGCTTGCCGCCACTACTACT-3′ |
| Reverse | 5′-GGAGCATCTGAGCAGAAGTAACAGA-3′ |
| Amplification (35 cycles) | 94°C (30 seconds), 58°C (30 seconds), 72°C (30 seconds) |
| Final elongation | 72°C (10 minutes) |
| IGF-IRβ (product size 450 bp) | |
| Forward | 5′-TGGGGAAGGAGTGCTGTAT-3′ |
| Reverse | 5′-CGGCCATCTCTGAATCATCTTG-3′ |
| Amplification (35 cycles) | 94°C (60 seconds), 56°C (60 seconds), 72°C (60 seconds) |
| Final elongation | 72°C (10 minutes) |
| IGF-I (product size 413 bp) | |
| Forward | 5′-TCCTCGCATCTCTTCTACC-3′ |
| Reverse | 5′-TGGCATGTCACTCTTCACT-3′ |
| Amplification (35 cycles) | 94°C (60 seconds), 58°C (60 seconds), 72°C (60 seconds) |
| Final elongation | 72°C (10 minutes) |
| IGF-I (product size 299 bp) | |
| Forward | 5′-GAAGGTGAAGATGCACACCA-3′ |
| Reverse | 5′-AGCGAGCTGACTTGGCAGGCTTGA-3′ |
| Amplification (35 cycles) | 94°C (60 seconds), 63°C (40 seconds), 72°C (60 seconds) |
| Final elongation | 72°C (10 minutes) |
| Primers and conditions . | . |
|---|---|
| IGF-IRα (product size 307 bp) | |
| Forward | 5′-GTAGCTTGCCGCCACTACTACT-3′ |
| Reverse | 5′-GGAGCATCTGAGCAGAAGTAACAGA-3′ |
| Amplification (35 cycles) | 94°C (30 seconds), 58°C (30 seconds), 72°C (30 seconds) |
| Final elongation | 72°C (10 minutes) |
| IGF-IRβ (product size 450 bp) | |
| Forward | 5′-TGGGGAAGGAGTGCTGTAT-3′ |
| Reverse | 5′-CGGCCATCTCTGAATCATCTTG-3′ |
| Amplification (35 cycles) | 94°C (60 seconds), 56°C (60 seconds), 72°C (60 seconds) |
| Final elongation | 72°C (10 minutes) |
| IGF-I (product size 413 bp) | |
| Forward | 5′-TCCTCGCATCTCTTCTACC-3′ |
| Reverse | 5′-TGGCATGTCACTCTTCACT-3′ |
| Amplification (35 cycles) | 94°C (60 seconds), 58°C (60 seconds), 72°C (60 seconds) |
| Final elongation | 72°C (10 minutes) |
| IGF-I (product size 299 bp) | |
| Forward | 5′-GAAGGTGAAGATGCACACCA-3′ |
| Reverse | 5′-AGCGAGCTGACTTGGCAGGCTTGA-3′ |
| Amplification (35 cycles) | 94°C (60 seconds), 63°C (40 seconds), 72°C (60 seconds) |
| Final elongation | 72°C (10 minutes) |