Length of VWF strings after cleavage by HUVEC ADAMTS-13
| . | 0-2 minutes . | 5 minutes . | 10-15 minutes . |
|---|---|---|---|
| Average length, μm | 107 ± 57 | 28 ± 36 | 19 ± 19 |
| Size range, μm | 11-248 | 2-205 | 2-107 |
| . | 0-2 minutes . | 5 minutes . | 10-15 minutes . |
|---|---|---|---|
| Average length, μm | 107 ± 57 | 28 ± 36 | 19 ± 19 |
| Size range, μm | 11-248 | 2-205 | 2-107 |
Histamine-stimulated HUVECs were incubated in Ca2+/Zn2+-containing buffer for 0 to 2, 5, and 10 to 15 minutes, fixed, and stained with rabbit anti–VWF plus goat anti–rabbit IgG-488. VWF string lengths (± SD) were measured from microscope views (201 μm × 150 μm) at ×200 at each incubation time during 10 experiments.