Comparisons of the functions of frozen versus fresh CD40-activated B cells
. | Frozen CD40-activated B cells (n = 10) . | Fresh CD40-activated B cells (n = 6) . | P . |
---|---|---|---|
Percent of induced CD4highCD25+ Tregs after 6 days of coculture | 65.30 ± 3.04 | 62.83 ± 3.25 | .6066 |
Absolute number (×106) of induced CD4highCD25+ Tregs from 106 precursors after 6 days of coculture | 0.84 ± 0.06 | 0.82 ± 0.09 | .8475 |
Percent of inhibition at a Treg/responder cell ratio of 1:64 | 51.75 ± 1.25 | 50.00 ± 1.07 | .3664 |
. | Frozen CD40-activated B cells (n = 10) . | Fresh CD40-activated B cells (n = 6) . | P . |
---|---|---|---|
Percent of induced CD4highCD25+ Tregs after 6 days of coculture | 65.30 ± 3.04 | 62.83 ± 3.25 | .6066 |
Absolute number (×106) of induced CD4highCD25+ Tregs from 106 precursors after 6 days of coculture | 0.84 ± 0.06 | 0.82 ± 0.09 | .8475 |
Percent of inhibition at a Treg/responder cell ratio of 1:64 | 51.75 ± 1.25 | 50.00 ± 1.07 | .3664 |