Table 1

Comparisons of the functions of frozen versus fresh CD40-activated B cells

Frozen CD40-activated B cells (n = 10)Fresh CD40-activated B cells (n = 6)P
Percent of induced CD4highCD25+ Tregs after 6 days of coculture 65.30 ± 3.04 62.83 ± 3.25 .6066 
Absolute number (×106) of induced CD4highCD25+ Tregs from 106 precursors after 6 days of coculture 0.84 ± 0.06 0.82 ± 0.09 .8475 
Percent of inhibition at a Treg/responder cell ratio of 1:64 51.75 ± 1.25 50.00 ± 1.07 .3664 
Frozen CD40-activated B cells (n = 10)Fresh CD40-activated B cells (n = 6)P
Percent of induced CD4highCD25+ Tregs after 6 days of coculture 65.30 ± 3.04 62.83 ± 3.25 .6066 
Absolute number (×106) of induced CD4highCD25+ Tregs from 106 precursors after 6 days of coculture 0.84 ± 0.06 0.82 ± 0.09 .8475 
Percent of inhibition at a Treg/responder cell ratio of 1:64 51.75 ± 1.25 50.00 ± 1.07 .3664 

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