Binding constants for prothrombinase and FXa-FVa assembly
| Cofactor species . | Kd, nM (+PCPS) . | Kd, nM (−PCPS) . |
|---|---|---|
| pt-rFV | 3.06 ± 0.45 | 8.08 ± 1.23 |
| pt-rFV-QQ | 2.88 ± 0.37 | 9.39 ± 1.54 |
| pt-rFVa | 3.84 ± 0.78 | 7.54 ± 0.62 |
| hFV-810 | 3.76 ± 0.87 | >100* |
| Cofactor species . | Kd, nM (+PCPS) . | Kd, nM (−PCPS) . |
|---|---|---|
| pt-rFV | 3.06 ± 0.45 | 8.08 ± 1.23 |
| pt-rFV-QQ | 2.88 ± 0.37 | 9.39 ± 1.54 |
| pt-rFVa | 3.84 ± 0.78 | 7.54 ± 0.62 |
| hFV-810 | 3.76 ± 0.87 | >100* |
The data are representative of 2 independent measurements. The errors in the fitted constants represent plus or minus 2 SDs. Equilibrium dissociation constants are inferred from kinetic experiments detailed in “Kinetics of protein substrate cleavage.”
We were not able to accurately determine a value; a lower limit estimate is provided.